Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,901,121	A→G	D202D (GAT→GAC)	pstB ←	phosphate ABC transporter ATP‑binding protein PstB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,901,121	0	A	G	100.0%	56.6 / NA	18	D202D (GAT→GAC)	pstB	phosphate ABC transporter ATP‑binding protein PstB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/6);  total (12/6)

TTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTC  >  NZ_CP009273/3901040‑3901192                                                                                  |                                                                        ttCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCtt                                                                 >  2:84815/1‑90 (MQ=255)    gCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAg                                                              >  1:367117/1‑90 (MQ=255)    gCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAg                                                              >  1:146338/1‑90 (MQ=255)              aTAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATc                                                    <  2:569625/90‑1 (MQ=255)                 aaCGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGACCTGCTTCAGTTCGGTGATCAGc                                                 <  1:71584/90‑1 (MQ=255)                   cGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctc                                               >  2:123488/1‑90 (MQ=255)                       gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCa                                           >  2:621284/1‑90 (MQ=255)                       gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCa                                           >  2:26949/1‑90 (MQ=255)                            gTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACg                                      >  2:404647/1‑90 (MQ=255)                               ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                   <  2:620295/90‑1 (MQ=255)                                   caacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                               <  2:462114/90‑1 (MQ=255)                                        cgcAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGAt                          <  2:55327/90‑1 (MQ=255)                                          cAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAg                        >  2:618040/1‑90 (MQ=255)                                                gctgcATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGa                  >  1:491901/1‑90 (MQ=255)                                                        ttgtGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGaa          >  2:130636/1‑90 (MQ=255)                                                            gggTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGc                                                 <  2:489438/47‑1 (MQ=255)                                                            gggTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGc                                                 >  1:489438/1‑47 (MQ=255)                                                                gACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTtc  >  1:331654/1‑90 (MQ=255)                                                                                  |                                                                        TTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTC  >  NZ_CP009273/3901040‑3901192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑