Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,269,793	G→C	intergenic (+37/‑115)	ghxP → / → yjcE	guanine/hypoxanthine transporter GhxP/Na+/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,269,793	0	G	C	100.0%	57.2 / NA	19	intergenic (+37/‑115)	ghxP/yjcE	guanine/hypoxanthine transporter GhxP/Na+/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/5);  total (14/5)

TATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGT  >  NZ_CP009273/4269708‑4269868                                                                                      |                                                                            tATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCg                                                                         >  1:322957/1‑90 (MQ=255)      ccGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGttttt                                                                    >  1:174118/1‑90 (MQ=255)      ccGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGttttt                                                                    >  2:648109/1‑90 (MQ=255)      ccGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGttttt                                                                    >  1:50455/1‑90 (MQ=255)      ccGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGttttt                                                                    >  2:533043/1‑90 (MQ=255)      ccGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGttttt                                                                    >  2:304847/1‑90 (MQ=255)        gTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTAt                                                                  >  1:257737/1‑90 (MQ=255)                 gTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTATTTTCTCCGc                                                         <  2:606058/90‑1 (MQ=255)                     ccTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTATTTTCTCCGCACAt                                                     <  1:648109/90‑1 (MQ=255)                     ccTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTATTTTCTCCGCACAt                                                     <  2:354403/90‑1 (MQ=255)                               gggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCt                                           >  2:525710/1‑90 (MQ=255)                                        ggCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGata                                  >  2:217433/1‑90 (MQ=255)                                            aTCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCt                              >  2:55388/1‑90 (MQ=255)                                                 aTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGaaaa                         <  1:267529/90‑1 (MQ=255)                                                      tCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATg                    >  2:476052/1‑90 (MQ=255)                                                      tCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATg                    >  2:495719/1‑90 (MQ=255)                                                      tCATCGGATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATg                    >  1:535431/1‑90 (MQ=255)                                                            gATTCTGAAAACGGGTGGCAATGGCTCCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAtt              >  2:552298/1‑90 (MQ=255)                                                                        gggTGGCAATGGCTCCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGt  <  2:557819/90‑1 (MQ=255)                                                                                      |                                                                            TATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGT  >  NZ_CP009273/4269708‑4269868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑