| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,903 | T→A | D27E (GAT→GAA) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,903 | 0 | T | A | 96.8% | 102.5 / NA | 32 | D27E (GAT→GAA) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base A (17/13); minor base C (0/1); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC > NZ_CP009273/49820‑49991 | tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc < 1:106912/90‑1 (MQ=255) gATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGtt < 1:488939/90‑1 (MQ=255) tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTa > 2:167533/1‑90 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaaa > 2:410537/1‑90 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGAAATCGCCTGGTTTaaa > 2:317924/1‑90 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa < 2:345428/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa < 2:308860/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa > 2:110908/1‑90 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAACTGCCTGCCGAACTCGCCTGGTTTAAACGCaa > 1:274794/1‑90 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt < 2:608459/90‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt > 2:64387/1‑90 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt > 1:141476/1‑90 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt > 1:401223/1‑90 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt > 1:227645/1‑90 (MQ=255) aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTtaaataa < 2:189147/90‑1 (MQ=255) gCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTtaaataaa < 1:110908/90‑1 (MQ=255) tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg > 2:118865/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 2:120147/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 2:229281/1‑90 (MQ=255) ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg < 1:437788/90‑1 (MQ=255) ttATCGGCATGGAAAACGCCATGCCCTGGAACCTGGCTGCCGAACTCGCCTGGTTTAAAAGCAAAACCTTAAATAAACCCGGGATTATgg > 2:497949/1‑90 (MQ=255) taccGGCATGGAAAACGCTATGCGGGGGACCCTGCGTGCCGACCTCGCCTGTTTTAAACGCAACCCCTTAAATACACCCGTGATTATggg < 2:169778/87‑1 (MQ=255) aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 2:248383/90‑1 (MQ=255) aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 1:334133/90‑1 (MQ=255) aaaaCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt < 2:204160/90‑1 (MQ=255) aaCGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc < 1:342602/36‑1 (MQ=255) aaCGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc > 2:342602/1‑36 (MQ=38) aCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa > 2:588042/1‑49 (MQ=255) aCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa < 1:588042/49‑1 (MQ=255) tGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa > 2:152487/1‑90 (MQ=255) gTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg > 1:17395/1‑90 (MQ=255) aTCGCGCCTGGTTTAAACGCAACACCTTAAATAACCCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc < 2:525589/90‑1 (MQ=255) | TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC > NZ_CP009273/49820‑49991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |