Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	502,414	T→C	D42G (GAT→GGT)	ybaK ←	Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase YbaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	502,414	0	T	C	100.0%	50.2 / NA	17	D42G (GAT→GGT)	ybaK	Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase YbaK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/6);  total (11/6)

ACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTAT  >  NZ_CP009273/502335‑502486                                                                                |                                                                         aCCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCaa                                                                >  1:338517/1‑90 (MQ=255)       gACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTaa                                                          >  2:65295/1‑90 (MQ=255)       gACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTaa                                                          >  2:547076/1‑90 (MQ=255)       gACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTaa                                                          >  2:483286/1‑90 (MQ=255)                                   ttCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTg                              >  2:93089/1‑90 (MQ=255)                                        atCACCGTTCACTGCCACCAGCAGCGTTCTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTc                         >  1:315734/1‑90 (MQ=255)                                         tCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCa                        >  2:276703/1‑90 (MQ=255)                                         tCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCa                        >  1:139646/1‑90 (MQ=255)                                           aCCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGc                      <  1:226240/90‑1 (MQ=255)                                              gTTCACTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCgg                   >  2:358280/1‑90 (MQ=255)                                                  aCTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGGCTTCATCGCCAAAATTGGTTTCAGCCGGATCg               <  2:133129/90‑1 (MQ=255)                                                  aCTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCg               <  2:407391/90‑1 (MQ=255)                                                  aCTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCg               <  1:290868/90‑1 (MQ=255)                                                   cTGCCACCAGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGt              >  1:312477/1‑90 (MQ=255)                                                         ccaGCAGCGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGt        <  2:528228/90‑1 (MQ=255)                                                          cagcagCGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACAACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTa       <  1:425114/90‑1 (MQ=255)                                                               gcGTTTTGTAGACCTGACCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTAt  >  2:52261/1‑90 (MQ=255)                                                                                |                                                                         ACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTAT  >  NZ_CP009273/502335‑502486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑