Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	609,731	A→G	D40G (GAT→GGT)	entF →	enterobactin non‑ribosomal peptide synthetase EntF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	609,731	0	A	G	100.0%	50.2 / NA	17	D40G (GAT→GGT)	entF	enterobactin non‑ribosomal peptide synthetase EntF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/11);  total (6/11)

CGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGA  >  NZ_CP009273/609648‑609806                                                                                    |                                                                            cGGCATCTGGATGGCAGAAAAACTGTCAGAATTCCCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGcc                                                                       <  2:408711/90‑1 (MQ=255) cGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGcc                                                                       <  2:511749/90‑1 (MQ=255) cGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGcc                                                                       <  1:274003/90‑1 (MQ=255) cGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGcc                                                                       >  1:602128/1‑90 (MQ=255)        tGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTg                                                                >  2:451947/1‑90 (MQ=255)               cAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGcc                                                                       >  1:225026/1‑76 (MQ=255)               cAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGcc                                                                       <  2:225026/76‑1 (MQ=255)                aGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCcgcgc                                                        <  1:228484/90‑1 (MQ=255)                               aTTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACt                                         <  1:452310/90‑1 (MQ=255)                                    ccTCCGCCTGGAGCGTGGCGCATTACGTTTAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAgcgc                                    <  1:200083/90‑1 (MQ=255)                                       ccGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAg                                 >  1:489414/1‑90 (MQ=255)                                       ccGCCTGGAGCGTGGCGCATTACGTTGAGTGAACCGGAGAGGATGGCGCGCCCTTACTGGCCCGCGCGGGGGTTGCCGGACCAGCGCacg                                 >  2:394421/1‑88 (MQ=255)                                        cGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGc                                <  1:599376/90‑1 (MQ=255)                                              gAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATAc                          <  1:254172/90‑1 (MQ=255)                                                gCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGc                        <  2:633370/90‑1 (MQ=255)                                                                tGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTAc        <  2:602128/90‑1 (MQ=255)                                                                      aaCCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGa  >  2:472752/1‑90 (MQ=255)                                                                                    |                                                                            CGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGA  >  NZ_CP009273/609648‑609806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑