Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	108,362	A→G	F242S (TTC→TCC)	zapD ←	cell division protein ZapD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	108,362	0	A	G	100.0%	76.8 / NA	24	F242S (TTC→TCC)	zapD	cell division protein ZapD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/8);  total (16/8)

CACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAT  >  NZ_CP009273/108281‑108444                                                                                  |                                                                                   cacaCCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGa                                                                            >  1:118495/1‑90 (MQ=255)  acacCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGAc                                                                           <  2:234459/90‑1 (MQ=255)              tCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCt                                                               <  2:373348/90‑1 (MQ=255)               cccGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTg                                                              >  2:324103/1‑90 (MQ=255)                  gCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGAc                                                                           >  2:413103/1‑74 (MQ=255)                  gCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGAc                                                                           <  1:413103/74‑1 (MQ=255)                     ggTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGtt                                                        <  1:136646/90‑1 (MQ=255)                         gggCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCa                                                    >  1:289999/1‑90 (MQ=255)                             aaTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAgtgt                                                <  2:217420/90‑1 (MQ=255)                               ttCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTcc                                              >  1:264317/1‑90 (MQ=255)                                  aCCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGc                                           <  2:289999/90‑1 (MQ=255)                                   ccGTAATAGTTTCTGACATCTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCg                                          >  1:629928/1‑90 (MQ=255)                                     gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGc                                        >  2:654648/1‑90 (MQ=255)                                             ttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACg                                >  1:50429/1‑90 (MQ=255)                                                               ttAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAg                                                   >  2:252453/1‑53 (MQ=255)                                                               ttAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAg                                                   <  1:252453/53‑1 (MQ=255)                                                               ttAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCtt              >  1:71546/1‑90 (MQ=255)                                                               ttAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCtt              >  1:110798/1‑90 (MQ=255)                                                                tAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTa             >  1:383206/1‑90 (MQ=255)                                                                       aGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCgg      >  2:199983/1‑90 (MQ=255)                                                                       aGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCgg      >  2:49014/1‑90 (MQ=255)                                                                       aGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGACATAAAACGAATGGCAAAAc                     >  2:304055/1‑75 (MQ=255)                                                                       aGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGACATAAAACGAATGGCAAAAc                     <  1:304055/75‑1 (MQ=255)                                                                           cAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAt  >  1:355203/1‑90 (MQ=255)                                                                                  |                                                                                   CACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAT  >  NZ_CP009273/108281‑108444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑