Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,174,665	T→G	L206W (TTG→TGG)	nagK →	N‑acetylglucosamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,174,665	0	T	G	100.0%	34.9 / NA	12	L206W (TTG→TGG)	nagK	N‑acetylglucosamine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/3);  total (9/3)

GCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTTGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTGGATTTATTA  >  NZ_CP009273/1174581‑1174747                                                                                     |                                                                                   gCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTGGCAgg                                                                               <  2:95782/90‑1 (MQ=255)           gCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTGGCAGGCTCCCGAAat                                                                     >  1:637506/1‑90 (MQ=255)              tGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTGGCAGGCTCCCGAAattat                                                                  >  2:66344/1‑90 (MQ=255)                                             ggTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTGGCAGGc                                                                              <  1:393310/47‑1 (MQ=255)                                             ggTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTGGCAGGc                                                                              >  2:393310/1‑47 (MQ=255)                                              gTTTTGCGTGGCTGTACCAACACTATTATCATCAACCGTGGCAGGCTCCCCAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCaa                                  <  2:434594/90‑1 (MQ=255)                                                                      attatCATCAACCGTGGCAGGCTCCCGAAATTGTTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTgg          >  2:59532/1‑90 (MQ=255)                                                                      attatCATCAACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTgg          >  2:222642/1‑90 (MQ=255)                                                                              caACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTGGATttatta  >  1:405912/1‑90 (MQ=255)                                                                              caACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTGGATttatta  >  1:406599/1‑90 (MQ=255)                                                                              caACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTGGATttatta  >  2:410149/1‑90 (MQ=255)                                                                              caACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTGGATttatta  >  2:437884/1‑90 (MQ=255)                                                                                     |                                                                                   GCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTTGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTGGATTTATTA  >  NZ_CP009273/1174581‑1174747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑