Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,394,663	T→C	G456G (GGA→GGG)	abgT ←	p‑aminobenzoyl‑glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,394,663	0	T	C	100.0%	49.1 / NA	16	G456G (GGA→GGG)	abgT	p‑aminobenzoyl‑glutamate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/5);  total (11/5)

AAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATA  >  NZ_CP009273/1394586‑1394726                                                                              |                                                                aaaaaGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGt                                                     >  1:383032/1‑90 (MQ=255)           gTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAg                                           <  2:600936/90‑1 (MQ=255)                       aGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGgcg                               >  1:533667/1‑90 (MQ=255)                          cTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTa                            <  1:529638/90‑1 (MQ=255)                          cTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTa                            <  2:383032/90‑1 (MQ=255)                            aaCGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTaaa                          >  1:45296/1‑90 (MQ=255)                                 aTAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCaa                     <  1:473199/90‑1 (MQ=255)                                 aTAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCaa                     >  2:117166/1‑90 (MQ=255)                                 aTAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCaa                     >  2:449969/1‑90 (MQ=255)                                  tAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAAt                    >  2:524633/1‑90 (MQ=255)                                    gtaagtaCCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATAc                  >  2:584794/1‑90 (MQ=255)                                              aGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCg        >  2:58703/1‑90 (MQ=255)                                                  tCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAg                         <  1:360680/69‑1 (MQ=255)                                                  tCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAg                         >  2:360680/1‑69 (MQ=255)                                                  tCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCaa    >  1:401443/1‑90 (MQ=255)                                                    gcgTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATa  >  1:536202/1‑90 (MQ=255)                                                                              |                                                                AAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATA  >  NZ_CP009273/1394586‑1394726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑