Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,434,377	A→G	F222S (TTC→TCC)	nifJ ←	pyruvate:ferredoxin (flavodoxin) oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,434,377	0	A	G	100.0%	35.3 / NA	12	F222S (TTC→TCC)	nifJ	pyruvate:ferredoxin (flavodoxin) oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/3);  total (9/3)

GGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTG‑‑GCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCAT  >  NZ_CP009273/1434298‑1434452                                                                                |                                                                              ggCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTc                                                                     <  1:580156/90‑1 (MQ=255)                 atCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTG‑‑GCGGACGTa                                                   >  2:606499/1‑90 (MQ=255)                        gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTG‑‑GCGGACGTACCGCGGa                                            >  1:544610/1‑90 (MQ=255)                                  gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGCGGACGTACCGCGGATCACCGGa                                    >  1:324210/1‑90 (MQ=255)                                  gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTG‑‑GCGGACGTACCGCGGATCACCGGATg                                  >  2:81718/1‑90 (MQ=255)                                            gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTG‑‑GCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                        >  1:375763/1‑90 (MQ=255)                                            gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTG‑‑GCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                        >  1:601056/1‑90 (MQ=255)                                            gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTG‑‑GCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                        >  2:533366/1‑90 (MQ=255)                                                   aCCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTG‑‑GCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGccc                 <  1:389936/90‑1 (MQ=255)                                                    ccATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTG‑‑GCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCg                >  1:104381/1‑90 (MQ=255)                                                              gtggCTTCGCGAGACTGGGAATAAGTGTCAGGATTG‑‑GCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGa      <  1:644157/90‑1 (MQ=255)                                                                  cTTCGCGAGACTGGGAATAAGTGTCAGGATTG‑‑GCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCAt  >  2:287406/1‑90 (MQ=255)                                                                                |                                                                              GGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTG‑‑GCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCAT  >  NZ_CP009273/1434298‑1434452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑