Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,454,774	A→G	D16G (GAT→GGT)	paaI →	hydroxyphenylacetyl‑CoA thioesterase PaaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,454,774	0	A	G	100.0%	57.3 / NA	19	D16G (GAT→GGT)	paaI	hydroxyphenylacetyl‑CoA thioesterase PaaI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/13);  total (6/13)

TTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCAC  >  NZ_CP009273/1454694‑1454854                                                                                 |                                                                                 ttGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTgcgc                                                                         >  2:363907/1‑90 (MQ=255)      gcgcCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAg                                                                    >  1:398813/1‑90 (MQ=255)        gcCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAgcg                                                                  >  2:184429/1‑90 (MQ=255)                  gcTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCg                                                        <  1:123622/90‑1 (MQ=255)                      cTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACAt                                                    <  1:358378/90‑1 (MQ=255)                             gCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCa                                             >  1:71234/1‑90 (MQ=255)                                         cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                 <  2:447124/90‑1 (MQ=255)                                         cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                 <  2:398813/90‑1 (MQ=255)                                           tAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCtt                               <  2:71234/90‑1 (MQ=255)                                           tAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCtt                               <  2:65161/90‑1 (MQ=255)                                              ggCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGc                            <  2:121069/90‑1 (MQ=255)                                               gCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCt                           >  1:201348/1‑90 (MQ=255)                                                         aTGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCa                 <  1:350531/90‑1 (MQ=255)                                                                 gCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTc         <  1:398743/90‑1 (MQ=255)                                                                   aaTGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCAc       <  2:310103/90‑1 (MQ=255)                                                                   aaTGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCAc       <  2:367986/90‑1 (MQ=255)                                                                    aTGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACt      <  2:201348/90‑1 (MQ=255)                                                                        aTGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGcac  >  1:61969/1‑90 (MQ=255)                                                                              aCGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGCAGTGACCATGACCGTCACTGc    <  1:199653/82‑1 (MQ=255)                                                                                 |                                                                                 TTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCAC  >  NZ_CP009273/1454694‑1454854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑