| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,502,076 | A→G | N347D (AAC→GAC) | rlhA → | 23S rRNA 5‑hydroxycytidine C2501 synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,502,076 | 0 | A | G | 100.0% | 43.1 / NA | 15 | N347D (AAC→GAC) | rlhA | 23S rRNA 5‑hydroxycytidine C2501 synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/9); total (6/9) | |||||||||||
TAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGG > NZ_CP009273/1501999‑1502156 | tAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTa < 1:455319/90‑1 (MQ=255) gAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAgtcgt > 2:535206/1‑90 (MQ=255) catcTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAAt > 1:64777/1‑90 (MQ=255) gATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTc > 1:173891/1‑90 (MQ=255) tGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGaa > 1:516004/1‑90 (MQ=255) gTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAAcc < 2:88250/90‑1 (MQ=255) ccGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGgag > 1:462558/1‑90 (MQ=255) tgcgCCACTGGCAAATGCCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCCTGCCAATACGGTCGAGAAAACCGgaga < 2:613055/87‑1 (MQ=255) cGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGgaga < 1:320259/90‑1 (MQ=255) cGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGgaga < 1:639856/90‑1 (MQ=255) cGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGgaga < 2:115952/90‑1 (MQ=255) cACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATc < 1:490480/90‑1 (MQ=255) aaaTGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACcg < 1:19278/90‑1 (MQ=255) ggCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCG‑GGAAACCGGAGAAAATCAGTACCGCGTCt < 2:41352/90‑1 (MQ=255) gATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTgg > 2:153857/1‑90 (MQ=255) | TAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGG > NZ_CP009273/1501999‑1502156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |