| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,505,430 | A→G | K391E (AAA→GAA) | ydcR → | PLP‑dependent aminotransferase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,505,430 | 0 | A | G | 84.6% | 24.5 / ‑2.9 | 13 | K391E (AAA→GAA) | ydcR | PLP‑dependent aminotransferase family protein |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (5/6); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAC > NZ_CP009273/1505343‑1505518 | gCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGgaa > 2:19447/1‑90 (MQ=255) ttCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtcatc < 1:59713/90‑1 (MQ=255) ttCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtcatc < 2:574864/90‑1 (MQ=255) gtcgCCCGCGTCGCCAGCTTGCGGAACGTAAAAAACGTGCCTGGCAGGCCCTGCCGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATa < 2:450590/90‑1 (MQ=255) cGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGt < 1:19544/90‑1 (MQ=255) aCGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGtt > 1:439634/1‑90 (MQ=255) ggcaggcaCTGCTGCTTTATCTGCCTGGGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGt < 2:15387/90‑1 (MQ=255) caCTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTATTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATg < 1:342364/90‑1 (MQ=255) caCTGCTGCGTTATCTGCCAGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATg > 2:345749/1‑90 (MQ=255) gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg > 2:58674/1‑90 (MQ=255) aTCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTaa > 1:454445/1‑90 (MQ=255) cctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTg > 2:257271/1‑90 (MQ=255) gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggcg > 2:507957/1‑90 (MQ=255) gAAAATTCATCATAATGACAGTGGTTATTTTCTCTGGTGGGACCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAc < 2:439634/90‑1 (MQ=255) | GCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAC > NZ_CP009273/1505343‑1505518 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |