Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,673,542	A→G	K287E (AAG→GAG)	ydgH →	DUF1471 family protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,673,542	0	A	G	100.0%	27.2 / NA	12	K287E (AAG→GAG)	ydgH	DUF1471 family protein YdgH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/9);  total (3/9)

TGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATC  >  NZ_CP009273/1673459‑1673617                                                                                    |                                                                            tgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAg                                                                       <  2:424047/90‑1 (MQ=255)             aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt                                                           >  1:310834/1‑90 (MQ=255)              cAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTa                                                          <  1:489342/90‑1 (MQ=255)               aGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                        <  1:169860/75‑1 (MQ=255)               aGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                        >  2:169860/1‑75 (MQ=255)                  aTCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACcac                                                      <  2:390607/90‑1 (MQ=255)                                          aaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGt                              <  1:111551/90‑1 (MQ=255)                                                cccGAAGCCCCTTATCAGGTGGCGAAACGGGCGGCGGAGAAAGGTGCTAAGTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGtaat                        <  2:348310/89‑1 (MQ=255)                                                       cagccTACCAGGTTGCGAACCGTGCCGCGGAGAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGa                 <  2:256989/87‑1 (MQ=255)                                                            tATCAGGTTGCGAACCGTGCCGGGGAAAAAGGTGCTAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc            <  1:475980/90‑1 (MQ=255)                                                                    tGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgcctg    >  1:256989/1‑87 (MQ=255)                                                                      cGAAACGTGCCGCGGAAAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc  <  1:265232/90‑1 (MQ=255)                                                                                    |                                                                            TGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATC  >  NZ_CP009273/1673459‑1673617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑