Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,021,663	(A)5→4	coding (1737/2055 nt)	helD →	DNA helicase IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,021,659	0	A	.	100.0%	101.7 / NA	24	coding (1733/2055 nt)	helD	DNA helicase IV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (13/11);  total (13/11)

TTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATC  >  NZ_CP009273/1021571‑1021744                                                                                         |                                                                                      ttGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGaa                                                                                      >  1:757782/1‑90 (MQ=255)           aGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAac                                                                           >  1:226988/1‑90 (MQ=255)                    gTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGcc                                                                  <  1:558/90‑1 (MQ=255)                           cAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTg                                                           >  2:454087/1‑90 (MQ=255)                             aaCCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCa                                                         >  1:154169/1‑90 (MQ=255)                             aaCCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCa                                                         >  1:794174/1‑90 (MQ=255)                                 ggAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGaaaagc                                                                                  >  1:1153743/1‑60 (MQ=255)                                 ggAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGaaaagc                                                                                  <  2:1153743/62‑3 (MQ=255)                                   aaGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGa                                                   <  2:1180101/90‑1 (MQ=255)                                            ttCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGAc                                          >  1:1086668/1‑90 (MQ=255)                                            ttCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGAc                                          >  1:1071026/1‑90 (MQ=255)                                                gATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATAACCAtt                                      >  1:940564/1‑90 (MQ=255)                                                 aTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGTTGGCCGAAGTTGCAAATCGACTTTATGACCATTc                                     <  2:794174/90‑1 (MQ=255)                                                   ccTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCAt                                   <  1:87701/90‑1 (MQ=255)                                                          cgTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCa                            >  1:861713/1‑90 (MQ=255)                                                           gTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCaa                           <  1:705385/90‑1 (MQ=255)                                                                 aTCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCa                     <  2:154169/90‑1 (MQ=255)                                                                      aTGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTc                                     <  2:1123538/69‑1 (MQ=255)                                                                      aTGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTc                                     >  1:1123538/1‑69 (MQ=255)                                                                       tGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGc               <  2:1071026/90‑1 (MQ=255)                                                                       tGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGc               <  2:226811/90‑1 (MQ=255)                                                                        gAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCg              >  2:112837/1‑90 (MQ=255)                                                                            ccTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGAtt          <  1:1162836/90‑1 (MQ=255)                                                                             cTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTa         >  2:890503/1‑90 (MQ=255)                                                                              tGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTAc        >  1:200098/1‑90 (MQ=255)                                                                                  aGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGtca    >  2:998479/1‑90 (MQ=255)                                                                                    ccTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGtcatc  <  1:77298/90‑1 (MQ=255)                                                                                         |                                                                                      TTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATC  >  NZ_CP009273/1021571‑1021744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑