Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,632,437	(T)7→6	intergenic (‑71/+8)	BW25113_RS08135 ← / ← BW25113_RS25620	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,632,431	0	T	.	100.0%	136.6 / NA	32	intergenic (‑65/+14)	BW25113_RS08135/BW25113_RS25620	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/13);  total (19/13)

TCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTT  >  NZ_CP009273/1632344‑1632507                                                                                        |                                                                             tCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGAttt                                                                            <  2:417937/90‑1 (MQ=255) tCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGAttt                                                                            <  1:642719/90‑1 (MQ=255)        ttGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGAttttttcatg                                                                     <  1:662737/90‑5 (MQ=255)          ggATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCatgat                                                                  <  2:538420/90‑1 (MQ=255)            atatTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGt                                                                <  2:1126631/90‑1 (MQ=255)                  aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  2:891859/90‑1 (MQ=255)                  aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  2:534656/90‑1 (MQ=255)                  aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  1:977957/90‑1 (MQ=255)                       tgatgGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTAt                                                     <  2:137455/90‑1 (MQ=255)                         atgGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTAtgt                                                   <  1:245518/90‑1 (MQ=255)                               ttAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTAt                                             >  1:623932/1‑90 (MQ=255)                                 aaCTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTAcc                                                            <  1:261102/73‑1 (MQ=255)                                 aaCTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTAcc                                                            >  2:261102/1‑73 (MQ=255)                                   cTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          >  2:966737/1‑73 (MQ=255)                                   cTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  1:966737/73‑1 (MQ=255)                                   cTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCAt                                         >  1:293895/1‑90 (MQ=255)                                   cTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCAt                                         >  1:1111385/1‑90 (MQ=255)                                    tCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATc                                        <  1:548123/90‑1 (MQ=255)                                     ccAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCg                                       >  2:357548/1‑90 (MQ=255)                                      cAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGa                                      >  2:77876/1‑90 (MQ=255)                                      cAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGa                                      >  1:521056/1‑90 (MQ=255)                                      cAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGa                                      >  2:544698/1‑90 (MQ=255)                                      cAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGa                                      >  2:530979/1‑90 (MQ=255)                                        gTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAAt                                    >  2:1185472/1‑90 (MQ=255)                                        gTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAAt                                    <  1:177852/90‑1 (MQ=255)                                              gCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGacac                              <  2:119104/90‑1 (MQ=255)                                                      tACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCaaa                      >  2:1040050/1‑90 (MQ=255)                                                      tACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCaaa                      >  2:843125/1‑90 (MQ=255)                                                      tACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCaaa                      >  1:1217112/1‑90 (MQ=255)                                                             gctgGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTc               >  1:952267/1‑90 (MQ=255)                                                                ggTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  2:506157/44‑1 (MQ=38)                                                                ggTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          >  1:506157/1‑44 (MQ=38)                                                                 gTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGa           >  2:95628/1‑90 (MQ=255)                                                                          tAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCtt  >  1:227142/1‑90 (MQ=255)                                                                          tAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCtt  >  2:331869/1‑90 (MQ=255)                                                                                        |                                                                             TCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTT  >  NZ_CP009273/1632344‑1632507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑