Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,812,807	(C)7→8	intergenic (‑50/+55)	celF ← / ← chbR	6‑phospho‑beta‑glucosidase/transcriptional regulator ChbR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,812,800	1	.	C	100.0%	113.5 / NA	34	intergenic (‑43/+62)	celF/chbR	6‑phospho‑beta‑glucosidase/transcriptional regulator ChbR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (20/14);  total (20/14)

GCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATT  >  NZ_CP009273/1812716‑1812884                                                                                      |                                                                                     gctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑ccccc                                                                                 >  1:210332/1‑90 (MQ=255) gctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑ccccc                                                                                 >  1:960149/1‑90 (MQ=255) gctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑ccccc                                                                                 >  1:534636/1‑90 (MQ=255) gctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑ccccc                                                                                 >  1:954647/1‑90 (MQ=255)   tCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑ccccccc                                                                               >  2:76026/1‑90 (MQ=255)    cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑cccccccc                                                                              <  2:46070/90‑2 (MQ=255)    cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑cccccccc                                                                              <  1:289892/90‑2 (MQ=255)    cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑cccccccc                                                                              <  1:581496/90‑2 (MQ=255)    cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑cccccccc                                                                              <  1:599078/90‑2 (MQ=255)       gCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑ccccccccgga                                                                           <  2:1213607/90‑5 (MQ=255)         caccaATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACa                                                                          <  2:184839/90‑1 (MQ=255)         caccaATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACa                                                                          <  1:109730/90‑1 (MQ=255)                 gTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAaataat                                                                  >  1:580957/1‑90 (MQ=255)                   gacgacTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATcc                                                                >  1:817502/1‑90 (MQ=255)                       acTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCa                                                            <  1:802121/90‑1 (MQ=255)                        cTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACa                                                                          >  1:707740/1‑75 (MQ=255)                        cTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACa                                                                          <  2:707740/75‑1 (MQ=255)                         ttttAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAAc                                                          <  2:817502/90‑1 (MQ=255)                         ttttAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAAc                                                          <  1:514832/90‑1 (MQ=255)                          tttAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACa                                                         >  1:919960/1‑90 (MQ=255)                              aTTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGAc                                                     <  1:688045/90‑1 (MQ=255)                              aTTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGAc                                                     <  2:910643/90‑1 (MQ=255)                                ttCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTTGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAg                                                   <  2:92432/90‑1 (MQ=255)                                               tctcCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCa                                    <  2:1216655/90‑1 (MQ=255)                                                    cTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGtat                               >  2:625595/1‑90 (MQ=255)                                                    cTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGtat                               >  2:439603/1‑90 (MQ=255)                                                            cAGAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACtt                       <  1:179111/90‑1 (MQ=255)                                                              gAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATAtg                                              >  2:1096873/1‑65 (MQ=255)                                                              gAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATAtg                                              <  1:1096873/65‑1 (MQ=255)                                                              gAATACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTAc                     >  2:270734/1‑90 (MQ=255)                                                               aaTACTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACt                    <  1:76026/90‑1 (MQ=255)                                                                   cTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGAtt                >  2:581760/1‑90 (MQ=255)                                                                   cTGATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGAtt                >  1:71985/1‑90 (MQ=255)                                                                     gATATCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTaa              >  2:902202/1‑90 (MQ=255)                                                                        atCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAg                                                   <  1:814508/50‑1 (MQ=255)                                                                        atCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAg                                                   >  2:814508/1‑50 (MQ=255)                                                                        atCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAAtt           >  2:368527/1‑90 (MQ=255)                                                                        atCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAAtt           >  2:1184626/1‑90 (MQ=255)                                                                        atCTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAAtt           >  1:1064144/1‑90 (MQ=255)                                                                          cTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCa         >  2:374613/1‑90 (MQ=255)                                                                          cTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCa         >  1:260760/1‑90 (MQ=255)                                                                          cTGGCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCa         >  2:914900/1‑90 (MQ=255)                                                                             gCATATCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTc      >  2:534762/1‑90 (MQ=255)                                                                                 atCTGCCCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAAtt  >  2:121230/1‑90 (MQ=255)                                                                                      |                                                                                     GCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATT  >  NZ_CP009273/1812716‑1812884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑