Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,074,9240AG60.9% 6.9 / 22.8 23D55G (GAT→GGT) putPsodium/proline symporter PutP
Reads supporting (aligned to +/- strand):  ref base A (0/9);  new base G (14/0);  total (14/9)
Fisher's exact test for biased strand distribution p-value = 1.22e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.74e-05
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAAT‑GGGGTTGCCGGGCGCTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCC  >  NZ_CP009273/1074847‑1075004
                                                                             |                                                                                  
gAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCggctg                                                                        <  1:625531/90‑1 (MQ=255)
   aaaCTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCggctggct                                                                     <  1:1107383/90‑1 (MQ=255)
               cTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGGGGGTGGGCGGTTAT‑gggggt                                                        >  2:894992/1‑88 (MQ=255)
                        gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGGGGGGGTGGGTGTTTAT‑GGGGGTGGCgggggg                                               >  2:383252/1‑87 (MQ=255)
                        gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGGGGGTGGGTGTTTAT‑GGGGGTGGCGGgggc                                               >  2:492525/1‑90 (MQ=255)
                        gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGCGGGGGGGGGTTTAT‑GGGGGTGGCgggggg                                               >  2:1001515/1‑87 (MQ=255)
                                       tGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGGTATGAGGGGGGGGGTGTTTAT‑GGGGGGGGCGGGGGCTG‑Ttttttttttccg                               >  2:242464/1‑86 (MQ=255)
                                       tGGGCCATTCGTGACGGCATTATCGGCGGGTGCGGCGGGTATGAGCGGGTGGCTGTTTAT‑GGGGGTGGCGGGGGCTG‑Ttttttttttccg                               >  2:534979/1‑86 (MQ=255)
                                       tGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATGAGGGGGTGGCTGTTTATGGGGGTTGCGGGGGGCGT‑TTTTTTTTTCCg                                >  2:457719/1‑90 (MQ=255)
                                       tGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGGTTTAGGGGGGGGGTGTTTAT‑TGGGGTGGCGGGGGCGG‑TTTTTTTTTTCgg                               >  2:935933/1‑90 (MQ=255)
                                       tGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGGATGAGGGGGTGGGGGTTTAT‑GGGGGTGCCGGGGGCGG‑GTTTTTTTTTCgg                               >  1:211355/1‑90 (MQ=255)
                                       tGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGGATGCGGGGGGGGGCGTTTAT‑GGGGGTGGGGGGGGCGG‑Tttttttttttgg                               >  1:1222898/1‑86 (MQ=255)
                                       tGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGCGGCTGGGTGTTTAT‑GGGGGTGCCGGGGGCTG‑Ttttttttttccg                               >  2:510197/1‑86 (MQ=255)
                                             aTTCGTGACGGCATTATCGGCGGGTGGGGCGGGTATGAGCGGCGGGGTGTTTAT‑GGGGGTGGCGGGGGCCG‑TTTTTTTTTTCGGGGTTTc                         >  2:143575/1‑90 (MQ=255)
                                             aTTCGTGACGGCATTATCGGCGGGGGGGGCGGGGATTAGGGGGGGGGGGGTTAT‑GGGGGTGGCGGGGGGTG‑GTTTTTTTTTCGGGGtttt                         >  2:320586/1‑89 (MQ=255)
                                             aTTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGGGGCGGGCGGGTTAT‑GGGGGTGGCGGGGGCGGTTTTTTTTTTCCGGGtttt                          >  2:836457/1‑90 (MQ=255)
                                                          ttATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAAT‑GGGGTTGCCGGGCGCTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATc            <  2:138932/90‑1 (MQ=255)
                                                          ttATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAAT‑GGGGTTGCCGGGCGCTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATc            <  2:779091/90‑1 (MQ=255)
                                                          ttATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAAT‑GGGGTTGCCGGGCGCTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATc            <  1:316406/90‑1 (MQ=255)
                                                               ggcggGTGCGTCGGATATGAGCGGCTGGCTGTTAAT‑GGGGTTGCCGGGCGCTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCAt       <  2:476062/90‑1 (MQ=255)
                                                               ggcggGTGCGTCGGATATGAGCGGCTGGCTGTTAAT‑GGGGTTGCCGGGCGCTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCAt       <  2:935384/90‑1 (MQ=255)
                                                                    gTGCGTCGGATATGAGCGGCTGGCTGTTAAT‑GGGGTTGCCGGGCGTTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGcc  <  1:615404/90‑1 (MQ=255)
                                                                    gTGCGTCGGATATGAGCGGCTGGCTGTTAAT‑GGGGTTGCCGGGCGCTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGcc  <  2:1222898/90‑1 (MQ=255)
                                                                             |                                                                                  
GAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAAT‑GGGGTTGCCGGGCGCTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCC  >  NZ_CP009273/1074847‑1075004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑