| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,331 | 0 | T | G | 56.2% | 10.3 / 13.2 | 16 | intergenic (+21/‑82) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (9/0); total (12/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAA > NZ_CP009273/2380243‑2380409 | gaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGtg > 1:143461/1‑89 (MQ=255) ttATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGaaaa < 2:33674/90‑1 (MQ=255) cAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCg < 1:1129819/90‑1 (MQ=255) aaaaTGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACa < 2:609239/90‑1 (MQ=255) tGGTGTTATAACTTTGTTATACAACAGTAAATAATAAATATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTc > 2:280938/1‑90 (MQ=255) gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAAAAAAAACGACAAGCACATTTTATTTTTCCTCAt > 2:57260/1‑90 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 2:820850/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTAATCTGTTTTTAg > 2:140462/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGGCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTGtttttaa > 2:1002038/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGtttttaa > 2:76574/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTTCTCtttttttaa > 2:1044368/1‑87 (MQ=255) acaGTAAATAATAAAAATTTCTCGAGGCGGGGCCGGAAAAAAAGACAAGGACAATTTATTTTTTCTCCTCTTTTTTTAAATGGGaaaaaa > 1:178076/1‑87 (MQ=255) taataaAAATTTCTCGAGGCGGGGCCAGAAAAAGAAAGAGGGACATTTTATTTTTCCTCCTCTCTTTTTAGATGGGAAAAAAGAAAATCa > 2:1181492/1‑90 (MQ=255) taataaAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAACATGAAAATCa > 2:363611/1‑90 (MQ=255) taataaAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTTTTTTTAAATGGGAAAAAAGaaaatta > 2:880690/1‑86 (MQ=255) ataaAAATTTCTCGAGGCGGGGGCAGAAAAAAAGAGATGCACATTTTTTTTTTCCCCATTTGTTTTTAAATGGGGAAAAAGAAAATTAAc > 2:607181/1‑90 (MQ=255) ctcGAGGCGGGGCCCGAAAAAAAGACATGCACATTTTTTTTTTTCTCATCTTTTTTTAGATGGGAAAAAAGAAAATTAAAAGAGGaaaaa > 1:982794/1‑90 (MQ=255) | GATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAA > NZ_CP009273/2380243‑2380409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |