| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,362 | 0 | C | T | 57.1% | ‑4.8 / 21.7 | 21 | intergenic (+52/‑51) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base C (7/2); new base T (12/0); total (19/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGA > NZ_CP009273/2380277‑2380450 | tGGTGTTATAACTTTGTTATACAACAGTAAATAATAAATATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTc > 2:280938/1‑90 (MQ=255) gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAAAAAAAACGACAAGCACATTTTATTTTTCCTCAt > 2:57260/1‑90 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 2:820850/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTAATCTGTTTTTAg > 2:140462/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGGCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTGtttttaa > 2:1002038/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGtttttaa > 2:76574/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTTCTCtttttttaa > 2:1044368/1‑87 (MQ=255) acaGTAAATAATAAAAATTTCTCGAGGCGGGGCCGGAAAAAAAGACAAGGACAATTTATTTTTTCTCCTCTTTTTTTAAATGGGaaaaaa > 1:178076/1‑87 (MQ=255) taataaAAATTTCTCGAGGCGGGGCCAGAAAAAGAAAGAGGGACATTTTATTTTTCCTCCTCTCTTTTTAGATGGGAAAAAAGAAAATCa > 2:1181492/1‑90 (MQ=255) taataaAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAACATGAAAATCa > 2:363611/1‑90 (MQ=255) taataaAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTTTTTTTAAATGGGAAAAAAGaaaatta > 2:880690/1‑86 (MQ=255) ataaAAATTTCTCGAGGCGGGGGCAGAAAAAAAGAGATGCACATTTTTTTTTTCCCCATTTGTTTTTAAATGGGGAAAAAGAAAATTAAc > 2:607181/1‑90 (MQ=255) ctcGAGGCGGGGCCCGAAAAAAAGACATGCACATTTTTTTTTTTCTCATCTTTTTTTAGATGGGAAAAAAGAAAATTAAAAGAGGaaaaa > 1:982794/1‑90 (MQ=255) cAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGa < 1:1037400/90‑1 (MQ=255) aaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt > 1:118911/1‑90 (MQ=255) aaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt > 2:558864/1‑90 (MQ=255) aaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt > 1:933125/1‑90 (MQ=255) cATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTg > 1:253558/1‑90 (MQ=255) ttttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTc > 2:896695/1‑90 (MQ=255) tCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGa > 1:988577/1‑90 (MQ=255) tCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGa > 1:897070/1‑90 (MQ=255) | TGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGA > NZ_CP009273/2380277‑2380450 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |