Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,501,8650AC63.2% 2.2 / 12.4 19L123V (TTG→GTG) fecDFe(3+) dicitrate ABC transporter permease subunit FecD
Reads supporting (aligned to +/- strand):  ref base A (5/2);  new base C (0/12);  total (5/14)
Fisher's exact test for biased strand distribution p-value = 1.81e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CAGGCTGGCCCAGCATGCAGAAAGCGCCACGCCGGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGG  >  NZ_CP009273/4501776‑4501953
                                                                                         |                                                                                        
cAGGCTGGCCCAGCATGCAGAAAGCGCCACGCCGGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCaa                                                                                          >  2:867088/1‑90 (MQ=255)
cAGGCTGGCCCAGCATGCAGAAAGCGCCACGCCGGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCaa                                                                                          >  2:426349/1‑90 (MQ=255)
cAGGCTGGCCCAGCATGCAGAAAGCGCCACGCCGGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCaa                                                                                          >  2:139463/1‑90 (MQ=255)
              aTGCAAAAAGCCCCCCGCCGGGGAGCCCCACCTCCATCGGCTGGTGGTTTTTTGCCAGCATCTTCAGCAATATCACCCCCGCCATgccgc                                                                            <  1:591732/90‑1 (MQ=255)
                gCAGAAAGCGCCACGCCGGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGccgccc                                                                          >  2:9735/1‑90 (MQ=255)
                    aaaGCGCCACGCCGGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCaa                                                                      <  2:140293/90‑1 (MQ=255)
                    aaaGCCCCCCGCGGGTGAGCGCCAGCTTCATCGGCTGGTGGGTTTTTGCCAGCATCTTCAGCAATATCACCCCCGCCATGCCGCCCGCaa                                                                      <  2:796158/90‑1 (MQ=255)
                             cgcgGGTGGGCGCCAGCTTCATCGGGTGGGGGGTTTTTGCCAGCATCTAAAGCAATACCACCCCCGCCATGCCGCCCGCAAAGGCcagca                                                             <  1:1057047/90‑1 (MQ=255)
                                       cgcCAGCTTCATCGGCTGGGGGGTCTTTGCCAGCATCTTCAGCAATATCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCAc                                                   <  1:239891/90‑1 (MQ=255)
                                       cgcCACCTGCACCGCCGGGGGGGTCTTCGCCGGCATCTCCAGAAATATCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCAc                                                   <  2:937546/90‑1 (MQ=255)
                                           aGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATc                                               >  2:401064/1‑90 (MQ=255)
                                                     gCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTg                                     >  1:804952/1‑90 (MQ=255)
                                                      cgggtggGTCTTTGCCAGCACCTTCAGCAATACCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGa                                    <  1:913701/88‑1 (MQ=255)
                                                       tggtggGTTTTTGCCAGCATCTTCAGCAATATCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGAc                                   <  2:184622/90‑1 (MQ=255)
                                                           gggTTTTTGCCAGCATCTTCAGCAATACCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCa                               <  1:842764/90‑1 (MQ=255)
                                                           gggTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCa                               <  2:654486/90‑1 (MQ=255)
                                                               cTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATaag                           >  2:1000497/1‑90 (MQ=255)
                                                                      aGCCTCTTAAGAAATACCCCCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTaga                    <  1:9735/90‑1 (MQ=255)
                                                                      aGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTaga                    >  1:934587/1‑90 (MQ=255)
                                                                      aGCATCTTCAGAAATATCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTaga                    <  2:1174646/90‑1 (MQ=255)
                                                                      aGCATCTTCACCAATATCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTaga                    <  2:779875/90‑1 (MQ=255)
                                                                           cTTCAGCAATATCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGcccc               <  1:1000497/90‑1 (MQ=255)
                                                                                        aaCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAgg  >  1:800407/1‑90 (MQ=255)
                                                                                         |                                                                                        
CAGGCTGGCCCAGCATGCAGAAAGCGCCACGCCGGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGG  >  NZ_CP009273/4501776‑4501953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: