Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,527,9260AC54.2% 19.5 / 30.3 24G219G (GGT→GGGnanMN‑acetylneuraminate epimerase
Reads supporting (aligned to +/- strand):  ref base A (8/3);  new base C (0/13);  total (8/16)
Fisher's exact test for biased strand distribution p-value = 2.24e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-05
Rejected as consensus: Frequency below/above cutoff threshold.

AATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAA‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATC  >  NZ_CP009273/4527841‑4528006
                                                                                       |                                                                                  
aaTTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCtt                                                                                <  1:39310/90‑1 (MQ=255)
aaTTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCtt                                                                                <  1:725364/90‑1 (MQ=255)
               aaTCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAA‑‑ccgccg                                                               >  1:602412/1‑90 (MQ=255)
                     gTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAA‑‑CCGCCGCACCAg                                                         >  1:1065954/1‑90 (MQ=255)
                     gTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAA‑‑CCGCCGCACCAg                                                         >  2:818252/1‑90 (MQ=255)
                      ttaaaaTACGGCACCCGTTCGCAACCCTGTTTTGGCTTCGCCATTAAAAAGCCAGTTTTTATCCCCTTTATTCACAA‑‑CCGCCGCACCAGc                                                        <  2:332206/87‑1 (MQ=255)
                      ttCAAATACGGCATCCTTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGTTTTTATCACCTTTATTCACAA‑‑CCGCCGCACCAGc                                                        <  2:24423/90‑1 (MQ=255)
                       taaaaTCCGGCTCCCTTCCGCAATCCTGTTTTGGTTTCGCCATAAATAAGCCAGTTTTTATCCCCTTTATTCACAA‑‑CCGCCGCACCAGcc                                                       <  2:1164196/88‑1 (MQ=255)
                       taaaaTACGCCATCCTTCCGAAACCCTGTTTTGGCTTCCCCATTAATAAGCCAGTTTTTATCCCTTTTATCCACAA‑‑CCGCCGCACCAGcc                                                       <  2:1008868/88‑1 (MQ=255)
                           aTACGGCCCCCGTCCGAAACCCTGGTTTGGCTCCCCCATTAATAAGCCAGGTTTTATCCCTTTTATTCACAA‑‑CCGCCGCACCAGCCGTTc                                                   <  2:310926/90‑1 (MQ=255)
                            tACGCCTTCCTTCCGCAATCCTGTTTGGGCTCCCCCTTAAAAAAGCCGGTTTTTACCCCTTTTATTCACAA‑‑CCGCCGCACCAGCCGTTcc                                                  <  2:613660/90‑1 (MQ=255)
                              cgccATCCGTCCCCACTCCTGTTTTGGTTCCCCCATTAAAAAGCCAGTTTTTATCCCCTTTATTCACAA‑‑CCGCCGCACCAGCCGTTCCGt                                                <  2:121101/87‑1 (MQ=255)
                                  aTCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAA‑‑CCGCCGCACCAGCCGTTCCGTACCa                                            >  1:343831/1‑90 (MQ=255)
                                             aTCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAA‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGc                                 >  1:514367/1‑90 (MQ=255)
                                               tcttgTTTTGCTTCCCCAATAAAAAACCCGGTTTTTACCCCTTTTATCCACAC‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                <  1:235195/86‑1 (MQ=255)
                                                  tgTTTTGCTTCCCCAATTAAAAAGCCGGGTTTTACCCCTTTTATCCACAC‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGc                             <  2:960158/89‑1 (MQ=255)
                                                            cGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAA‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt                  >  2:1170737/1‑90 (MQ=255)
                                                            cGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAA‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt                  >  1:800964/1‑90 (MQ=255)
                                                               cctttAAAAAGCCGGGTTTTATCCCCTTTATTCACAA‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc                <  2:2998/87‑1 (MQ=255)
                                                                 aTTAATAAGCCAGTTTTTATCCCCTTTTTTCACAA‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtg              <  2:343831/90‑1 (MQ=255)
                                                                           cAGGTTTTATCACCTTTATTCACAA‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGa    >  2:70991/1‑90 (MQ=255)
                                                                             ggtttttttCCCCTTTTTTCCCAAACCCGCCGCCCCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAggg     <  1:868634/87‑1 (MQ=255)
                                                                              gtttttATCCCTTTTTTCCACAC‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc  <  2:852277/88‑1 (MQ=255)
                                                                              gtttttACCCCTTTTATCCACAC‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc  <  1:1161058/88‑1 (MQ=255)
                                                                                       |                                                                                  
AATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATTCACAA‑‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATC  >  NZ_CP009273/4527841‑4528006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: