Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,736	A→T	intergenic (+105/‑87)	kefC → / → folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,736	0	A	T	100.0%	181.6 / NA	47	intergenic (+105/‑87)	kefC/folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/21);  total (26/21)

ACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAT  >  NZ_CP009273/49650‑49818                                                                                       |                                                                                   acacTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAtttt                                                                                 <  2:761498/90‑1 (MQ=255) acacTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAtttt                                                                                 >  2:364213/1‑90 (MQ=255)  cacTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATtttt                                                                                >  2:676247/1‑90 (MQ=255)    cTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTcc                                                                              <  2:298039/90‑1 (MQ=255)     tttACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCt                                                                             <  1:1168065/90‑1 (MQ=255)       tACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCa                                                                           <  2:541717/90‑1 (MQ=255)            cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc                                                                      <  2:211442/90‑1 (MQ=255)            cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc                                                                      >  1:369955/1‑90 (MQ=255)              aaCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatcat                                                                    >  1:1115172/1‑90 (MQ=255)                cTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATcc                                                                  >  2:995564/1‑90 (MQ=255)                cTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATcc                                                                  >  2:432572/1‑90 (MQ=255)                    ttgtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGc                                                              <  2:608327/90‑1 (MQ=255)                        ttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCa                                                          >  2:735878/1‑90 (MQ=255)                          ttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAtttt                                                                                 <  2:320059/65‑1 (MQ=255)                          ttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAtttt                                                                                 >  1:320059/1‑65 (MQ=255)                                cATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga                                                  <  1:689511/90‑1 (MQ=255)                                cATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga                                                  <  1:1238254/90‑1 (MQ=255)                                      aaTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGttt                                            <  1:678971/90‑1 (MQ=255)                                         gcggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACg                                         <  2:731713/90‑1 (MQ=255)                                           ggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCt                                       <  2:851415/90‑1 (MQ=255)                                            gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatca                                                                     <  1:284797/59‑1 (MQ=255)                                            gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatca                                                                     >  2:284797/1‑59 (MQ=255)                                            gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt                                      >  1:672307/1‑90 (MQ=255)                                            gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt                                      <  2:672216/90‑1 (MQ=255)                                            gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt                                      <  2:1153257/90‑1 (MQ=255)                                              gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa                                    >  1:135620/1‑90 (MQ=255)                                              gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa                                    >  2:584319/1‑90 (MQ=255)                                              gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa                                    >  2:244551/1‑90 (MQ=255)                                               aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTAc                                   <  2:824696/90‑1 (MQ=255)                                                gTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga                                                  >  1:363659/1‑74 (MQ=255)                                                gTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga                                                  <  2:363659/74‑1 (MQ=255)                                                     gggAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAg                             >  2:538735/1‑90 (MQ=255)                                                          agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg                        >  2:324958/1‑90 (MQ=255)                                                          agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg                        >  2:438536/1‑90 (MQ=255)                                                          agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg                        >  2:1139013/1‑90 (MQ=255)                                                          agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg                        >  2:652303/1‑90 (MQ=255)                                                            agCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGAc                      >  1:1060647/1‑90 (MQ=255)                                                             gCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACa                     >  1:1170332/1‑90 (MQ=255)                                                              cGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACaa                    >  1:62776/1‑90 (MQ=255)                                                               gTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAt                   >  2:148089/1‑90 (MQ=255)                                                                tGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt                                      >  2:51287/1‑70 (MQ=255)                                                                tGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt                                      <  1:51287/70‑1 (MQ=255)                                                                tGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAtt                  >  2:210418/1‑90 (MQ=255)                                                                         cagcagAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATc         <  1:57380/90‑1 (MQ=255)                                                                           gcagAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCgg       >  2:272852/1‑90 (MQ=255)                                                                             agAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa     <  1:58687/90‑1 (MQ=255)                                                                              gAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa    <  2:1044395/90‑1 (MQ=255)                                                                              gAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa    <  1:57603/90‑1 (MQ=255)                                                                                atatAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAt  <  2:62776/90‑1 (MQ=255)                                                                                atatAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAt  >  1:886005/1‑90 (MQ=255)                                                                                atatAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAt  >  1:399353/1‑90 (MQ=255)                                                                                       |                                                                                   ACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAT  >  NZ_CP009273/49650‑49818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑