Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,840,928	T→C	E97G (GAA→GGA)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,840,928	0	T	C	100.0%	126.3 / NA	37	E97G (GAA→GGA)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/18);  total (19/18)

CGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCG  >  NZ_CP009273/1840849‑1841009                                                                                |                                                                                  cGGTGCCAGTTGCAGATAGTCCAGCCCTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGAGTGAACGATTCCGCTGGCTTc                                                                         <  1:1229346/90‑1 (MQ=255) cGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTc                                                                         <  2:856754/90‑1 (MQ=255) cGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTc                                                                         <  1:382612/90‑1 (MQ=255) cGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTc                                                                         <  1:568775/90‑1 (MQ=255)         gTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGa                                                                 <  2:724463/90‑1 (MQ=255)                   gTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTtgat                                                       <  2:1016150/90‑1 (MQ=255)                   gTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGGTtgat                                                       >  1:27275/1‑90 (MQ=255)                           cTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTaa                                               >  1:203795/1‑90 (MQ=255)                             tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                             >  2:480809/1‑90 (MQ=255)                             tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                             >  1:910878/1‑90 (MQ=255)                             tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                             >  1:70027/1‑90 (MQ=255)                             tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                             >  1:576143/1‑90 (MQ=255)                             tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                             >  1:380626/1‑90 (MQ=255)                             tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                             >  1:30749/1‑90 (MQ=255)                             tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATACCGCTGGCTTc                                                                         <  1:710081/62‑1 (MQ=255)                             tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATACCGCTGGCTTc                                                                         >  2:710081/1‑62 (MQ=255)                                 ccaccaGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgttgtt                                         <  1:1026837/90‑1 (MQ=255)                                  caccaGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGttt                                        <  1:491524/90‑1 (MQ=255)                                  caccaGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGttt                                        <  2:110514/90‑1 (MQ=255)                                  caccaGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGttt                                        <  2:684648/90‑1 (MQ=255)                                       gCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCa                                   >  2:669178/1‑90 (MQ=255)                                          aTTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACgg                                >  2:820698/1‑90 (MQ=255)                                           ttGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGa                               <  1:480809/90‑1 (MQ=255)                                                cTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTc                          <  2:1108496/90‑1 (MQ=255)                                                cTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTc                          <  2:123887/90‑1 (MQ=255)                                                 ttCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCg                         >  2:966837/1‑90 (MQ=255)                                                     cGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGc                     <  2:49558/90‑1 (MQ=255)                                                        tCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                  >  2:86282/1‑90 (MQ=255)                                                            ggTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAAtt              >  1:1071910/1‑90 (MQ=255)                                                               ccccGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGcc           >  1:894916/1‑90 (MQ=255)                                                                 ccGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGa                               >  2:1106179/1‑68 (MQ=255)                                                                 ccGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGa                               <  1:1106179/68‑1 (MQ=255)                                                                 ccGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCAc         >  1:228431/1‑90 (MQ=255)                                                                  cGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACt        <  1:478956/90‑1 (MQ=255)                                                                  cGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACt        <  2:933819/90‑1 (MQ=255)                                                                      gTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc    >  2:247552/1‑90 (MQ=255)                                                                        gAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCg  >  2:855095/1‑90 (MQ=255)                                                                                |                                                                                  CGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCG  >  NZ_CP009273/1840849‑1841009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑