Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,900,885	A→G	S145P (TCC→CCC)	rlmA ←	23S rRNA (guanine(745)‑N(1))‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,900,885	0	A	G	100.0%	105.6 / NA	32	S145P (TCC→CCC)	rlmA	23S rRNA (guanine(745)‑N(1))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/9);  total (23/9)

CCAGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAA  >  NZ_CP009273/1900799‑1900968                                                                                       |                                                                                    ccAGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGgaaa                                                                                  <  2:594944/90‑1 (MQ=255)   aGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTAGACGCCGCGTAAATACGCATTATGCCGTCCATACTGGTATCGGGAAACg                                                                                >  1:1043722/1‑90 (MQ=255)    gccgccGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACgg                                                                               >  2:293404/1‑90 (MQ=255)                    actCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGaa                                                               >  1:40375/1‑90 (MQ=255)                       cGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                            >  2:156405/1‑90 (MQ=255)                       cGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                            <  1:80559/90‑1 (MQ=255)                         tGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGac                                                          >  1:1099490/1‑90 (MQ=255)                            tAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGAcaca                                                       <  2:388316/90‑1 (MQ=255)                                       cTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCt                                            <  2:40375/90‑1 (MQ=255)                                         ttACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGc                                          >  1:1063522/1‑90 (MQ=255)                                         ttACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGc                                          >  2:1111901/1‑90 (MQ=255)                                              cGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATa                                     >  2:1057503/1‑90 (MQ=255)                                                gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGac                                                          <  1:515561/67‑1 (MQ=255)                                                gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGac                                                          >  2:515561/1‑67 (MQ=255)                                                gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGc                                   >  1:189850/1‑90 (MQ=255)                                                          aCGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTcgccgc                         >  1:487617/1‑90 (MQ=255)                                                                 atGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCtttt                  >  1:489500/1‑90 (MQ=255)                                                                  tGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTa                 >  2:597997/1‑90 (MQ=255)                                                                     cGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGAcc                                             <  1:171675/59‑1 (MQ=255)                                                                     cGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGAcc                                             >  2:171675/1‑59 (MQ=255)                                                                      gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGAcc                                             >  1:676169/1‑58 (MQ=255)                                                                      gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGAcc                                             <  2:676169/58‑1 (MQ=255)                                                                      gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc             >  2:328123/1‑90 (MQ=255)                                                                      gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc             >  2:998561/1‑90 (MQ=255)                                                                      gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc             >  2:1099887/1‑90 (MQ=255)                                                                      gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc             >  1:917300/1‑90 (MQ=255)                                                                      gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc             >  1:913579/1‑90 (MQ=255)                                                                      gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc             >  1:237926/1‑90 (MQ=255)                                                                        ccATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTa           <  2:642192/90‑1 (MQ=255)                                                                            aCTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCtt       <  1:962964/90‑1 (MQ=255)                                                                               ggTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGa    >  1:721531/1‑90 (MQ=255)                                                                               ggTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGa    >  1:160067/1‑90 (MQ=255)                                                                                 tATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGaaa  <  1:1099887/90‑1 (MQ=255)                                                                                       |                                                                                    CCAGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAA  >  NZ_CP009273/1900799‑1900968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑