Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,934,547	T→C	intergenic (‑97/+23)	lpxM ← / ← mepM	lauroyl‑Kdo(2)‑lipid IV(A) myristoyltransferase/murein DD‑endopeptidase MepM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,934,547	0	T	C	100.0%	99.2 / NA	30	intergenic (‑97/+23)	lpxM/mepM	lauroyl‑Kdo(2)‑lipid IV(A) myristoyltransferase/murein DD‑endopeptidase MepM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/13);  total (17/13)

TTTCGGATAAGGCAAAAATCAATCTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGTACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTGGCCTGCGCCAGGAATTCGCGACGATC  >  NZ_CP009273/1934461‑1934629                                                                                       |                                                                                   tttCGGATAAGGCAAAAATCAATCTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCAcc                                                                                 <  2:702385/90‑1 (MQ=255)       aTAAGGCAAAAATCAATCTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGc                                                                           <  2:668334/90‑1 (MQ=255)          aGGCAAAAATCAATCTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGaa                                                                        >  1:168247/1‑90 (MQ=255)          aGGCAAAAATCAATCTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGaa                                                                        >  2:274213/1‑90 (MQ=255)           ggCAAAAATCAATCTGGTGATANTGTAGAGGCGCAACTTGCCCCGCAACAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAAt                                                                       <  2:343869/90‑1 (MQ=255)                     aatcTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGttaat                                                             <  2:807491/90‑1 (MQ=255)                     aatcTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGttaat                                                             >  1:674809/1‑90 (MQ=255)                          ggTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATc                                                        <  2:415181/90‑1 (MQ=255)                            tGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCaa                                                      <  2:151870/90‑1 (MQ=255)                                   gtAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAg                                               >  2:165879/1‑90 (MQ=255)                                    tAGCGGCGCAACTTGCCCCGCACCAAATAAAAAGAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTa                                                >  2:805348/1‑88 (MQ=255)                                    tAGCGGCGCAACTTGCCCCGCACCAAATAAAAAGAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTa                                                <  1:805348/88‑1 (MQ=255)                                    tAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGc                                              >  2:99725/1‑90 (MQ=255)                                     aGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCt                                             >  2:645338/1‑90 (MQ=255)                                       cggcgCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGc                                           <  1:645338/90‑1 (MQ=255)                                         gcgcAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCgg                                         >  2:148725/1‑90 (MQ=255)                                         gcgcAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCgg                                         >  2:888468/1‑90 (MQ=255)                                         gcgcAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCgg                                         >  1:678914/1‑90 (MQ=255)                                           gcAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGca                                       >  1:1002268/1‑90 (MQ=255)                                           gcAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGca                                       >  1:97092/1‑90 (MQ=255)                                           gcAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGca                                       >  2:1130060/1‑90 (MQ=255)                                              aCTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACaa                                    <  2:678914/90‑1 (MQ=255)                                               cTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAAt                                   <  1:352615/90‑1 (MQ=255)                                                       gCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTgg                           >  2:842735/1‑90 (MQ=255)                                                          ccAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTGGCCt                        <  1:1193927/90‑1 (MQ=255)                                                          ccAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGCAGCTGCGGCACAATCTCTTTGGCCt                        <  2:493563/90‑1 (MQ=255)                                                                                gactgCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACCATCTCTTTGGCCTGGGACAGGAATTCCCGACGATc  >  1:1180586/1‑90 (MQ=255)                                                                                gactgCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAAt                                   <  2:375582/57‑1 (MQ=255)                                                                                gactgCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAAt                                   >  1:375582/1‑57 (MQ=255)                                                                                gactgCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTGGCCTGCGCCAGGAATTCGCGACGATc  >  1:866762/1‑90 (MQ=255)                                                                                       |                                                                                   TTTCGGATAAGGCAAAAATCAATCTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGTACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTGGCCTGCGCCAGGAATTCGCGACGATC  >  NZ_CP009273/1934461‑1934629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑