Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,965,782	T→G	E389D (GAA→GAC)	tar ←	methyl‑accepting chemotaxis protein II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,965,782	0	T	G	100.0%	119.0 / NA	36	E389D (GAA→GAC)	tar	methyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/21);  total (15/21)

AGGGCTTTGATCTCTTTTGCCGCCTGGGCGCTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGTTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTGGAAGGCAATACCGTCGATAAC  >  NZ_CP009273/1965696‑1965856                                                                                       |                                                                           aGGGCTTTGATCTCTTTTGCCGCCTGGGCGCTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCa                                                                         >  1:805843/1‑90 (MQ=255)   ggCTTTGATCTCTTTTGCCGCCTGGGCGCTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCAcc                                                                       >  2:1082182/1‑90 (MQ=255)            ctctTTTGCCGCCTGGGCGCTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCAcgcgc                                                              >  1:332040/1‑90 (MQ=255)            ctctTTTGCCGCCTGGGCGCTGCAACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCAcgcgc                                                              >  1:969015/1‑90 (MQ=255)            ctctTTGGCCGCCTGGGCGCGGCGACGGGCAAGATTCCCCCCTCCCCCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCAcgcgc                                                              <  1:732606/90‑1 (MQ=21)             tctTTTGCCGCCGGGGCGCTGCGACTGGCAAGATTCCGCCCTTCCCCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCAcgcgcg                                                             <  1:559190/90‑1 (MQ=255)              ctTTTGCCGCCTGGGCGCTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCgg                                                            >  1:1005156/1‑90 (MQ=255)              ctTTTGCCGCCTGGGCGCTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCgg                                                            >  1:1221066/1‑90 (MQ=255)                 ttcccgccgGGGCGCTGCGCCTGGCAAGATTCCGCCCTTCCCCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCtt                                                         <  1:546035/87‑1 (MQ=18)                   gccgccTGGGCGCTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCa                                                       >  1:721970/1‑90 (MQ=255)                        cTGGGCGCTGCGCCTGGCAAGATTACGCACTTCCCCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACcgc                                                  <  2:21027/90‑1 (MQ=21)                        cTGGGCGCTGCGACTGGCAAGATTACGCACTTCCCCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACcgc                                                  <  2:490820/90‑1 (MQ=255)                               cTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTc                                           <  2:805843/90‑1 (MQ=255)                                tGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCa                                          >  2:208902/1‑90 (MQ=255)                                 gCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCAcgcgc                                                              <  2:954003/69‑1 (MQ=255)                                 gCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCAcgcgc                                                              >  1:954003/1‑69 (MQ=255)                                    aCTGGCAAGATTACGCACTTCCCCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAgcgc                                      <  2:958861/90‑1 (MQ=255)                                     cTGGCAAGATTACGCCCTTCCCCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGcgcg                                     <  1:636894/90‑1 (MQ=255)                                     cTGGCAAGATTACGCACTTCCCCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGcgcg                                     <  2:323532/90‑1 (MQ=255)                                     cTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGcgcg                                     <  2:1005156/90‑1 (MQ=255)                                      tGGCAAGATTCCCCCCTCCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGa                                    <  1:331256/90‑1 (MQ=37)                                                aCGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGt                          >  1:101048/1‑90 (MQ=255)                                                    cccgcACCCGCCCCCACGGCAAAACCACGGCCCTGGTCACCCGCCCGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTgg                      <  1:847755/86‑1 (MQ=255)                                                    aCTTCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTgg                      <  2:1221066/90‑1 (MQ=255)                                                      ttCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTGGaa                    >  1:648157/1‑90 (MQ=255)                                                      ttCACCCGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTGGaa                    <  1:228893/90‑1 (MQ=255)                                                         ccccGCCACCACGGCGAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTGGAAGGc                 <  2:500547/89‑1 (MQ=255)                                                           ccGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTGGAAGGCaa               <  2:519569/90‑1 (MQ=255)                                                           ccGCCACCACGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTGGAAGGCaa               <  1:801532/90‑1 (MQ=255)                                                               caccacGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAg                                   >  1:657833/1‑66 (MQ=255)                                                               caccacGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAg                                   <  2:657833/66‑1 (MQ=255)                                                               caccacGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATAtt                             >  1:611227/1‑72 (MQ=255)                                                               caccacGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATAtt                             <  2:611227/72‑1 (MQ=255)                                                               caccacGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTGGAAGGCAATAcc           >  2:470523/1‑90 (MQ=255)                                                                  cacGGCAAAACCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTGGAAGGCAATACCGTc        >  1:899778/1‑90 (MQ=255)                                                                        aaaaCCACGGCCCTGGTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTGGAAGGCAATACCGTCGATAAc  <  2:912152/90‑1 (MQ=255)                                                                                       |                                                                           AGGGCTTTGATCTCTTTTGCCGCCTGGGCGCTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGGCAAAACCACGGCCCTGTTCACCCGCACGCGCGGCTTCAACCGCGGCATTCAGCGCGAGGATATTAGTCTGGAAGGCAATACCGTCGATAAC  >  NZ_CP009273/1965696‑1965856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑