Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,064,259	T→G	V93G (GTG→GGG)	BW25113_RS10440 →	GTPase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,064,259	0	T	G	100.0%	201.2 / NA	58	V93G (GTG→GGG)	BW25113_RS10440	GTPase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/34);  total (24/34)

TACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCAC  >  NZ_CP009273/2064172‑2064339                                                                                        |                                                                                 tACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGc                                                                                <  1:837271/90‑1 (MQ=255) tACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGc                                                                                <  1:1189513/90‑1 (MQ=255) tACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGc                                                                                <  2:500899/90‑1 (MQ=255) tACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGc                                                                                <  2:100165/90‑1 (MQ=255) tACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGc                                                                                <  1:596315/90‑1 (MQ=255)     gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt                                                                            >  2:706062/1‑90 (MQ=255)     gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt                                                                            >  2:126653/1‑90 (MQ=255)      tgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGttt                                                                           <  2:1017347/90‑1 (MQ=255)          tGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTgtgg                                                                       >  2:335808/1‑90 (MQ=255)             ttAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGa                                                                    >  1:301962/1‑90 (MQ=255)             ttAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGa                                                                    >  1:301963/1‑90 (MQ=255)                   ccGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAgg                                                              <  1:1149323/90‑1 (MQ=255)                     gATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGcc                                                            <  1:126653/90‑1 (MQ=255)                     gATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGGGACGCAGGcc                                                            >  2:847070/1‑90 (MQ=255)                          ccGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACaa                                                       <  2:1071302/90‑1 (MQ=255)                          ccGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACaa                                                       <  2:1012660/90‑1 (MQ=255)                                   gtctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGcc                                              >  2:808881/1‑90 (MQ=255)                                    tctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                             <  2:754824/90‑1 (MQ=255)                                    tctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                             <  2:904403/90‑1 (MQ=255)                                    tctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                             <  2:636354/90‑1 (MQ=255)                                       gtgGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGc                                          >  2:311442/1‑90 (MQ=255)                                        tgGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGGGGTGACGCAGGCCGACAAAACGGAGCCCTGcc                                         >  2:677815/1‑90 (MQ=255)                                         ggATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCa                                        <  1:1052904/90‑1 (MQ=255)                                          gATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCCGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCAt                                       <  1:677815/90‑1 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACGTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                    >  2:1044976/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCAt                                       >  2:389349/1‑87 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCAt                                       <  1:389349/87‑1 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                    >  2:727440/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                    >  2:734748/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                    >  2:751433/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                    >  2:825346/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                    >  2:932261/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                    >  2:1193773/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                    >  1:471072/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                    >  1:9791/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                    >  1:344410/1‑90 (MQ=255)                                              aGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAAt                                   <  2:716988/90‑1 (MQ=255)                                                      tGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATg                           <  1:706062/90‑1 (MQ=255)                                                       ggCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTgtggt                                                                      <  1:688094/46‑1 (MQ=255)                                                       ggCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTgtggt                                                                      >  2:688094/1‑46 (MQ=255)                                                         cGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGcc                        <  2:344410/90‑1 (MQ=255)                                                         cGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGcc                        <  2:816585/90‑1 (MQ=255)                                                         cGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGcc                        >  1:89725/1‑90 (MQ=255)                                                         cGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGcc                        <  2:9791/90‑1 (MQ=255)                                                          gACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCg                       <  2:356502/90‑1 (MQ=255)                                                                tCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTc                 <  1:795616/90‑1 (MQ=255)                                                                     cAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt            <  2:279650/90‑1 (MQ=255)                                                                     cAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt            <  1:665075/90‑1 (MQ=255)                                                                     cAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt            <  2:1179368/90‑1 (MQ=255)                                                                       gTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtc          <  1:825346/90‑1 (MQ=255)                                                                        tGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtct         >  1:600923/1‑90 (MQ=255)                                                                            gACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt            >  2:418936/1‑83 (MQ=255)                                                                            gACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt            <  1:418936/83‑1 (MQ=255)                                                                               aTCAGCAGGGGTTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac  <  2:990362/90‑1 (MQ=255)                                                                               aTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac  <  1:808881/90‑1 (MQ=255)                                                                               aTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac  <  1:340329/90‑1 (MQ=255)                                                                               aTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac  <  2:64260/90‑1 (MQ=255)                                                                               aTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac  <  2:271158/90‑1 (MQ=255)                                                                                        |                                                                                 TACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCAC  >  NZ_CP009273/2064172‑2064339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑