Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,434,529	A→C	F186V (TTT→GTT)	fabB ←	beta‑ketoacyl‑ACP synthase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,434,529	0	A	C	100.0%	170.5 / NA	50	F186V (TTT→GTT)	fabB	beta‑ketoacyl‑ACP synthase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/30);  total (20/30)

TGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGAGCTGATGGAGT  >  NZ_CP009273/2434445‑2434615                                                                                     |                                                                                       tgtCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGa                                                                                   <  2:335762/90‑1 (MQ=255)          aTTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGtt                                                                          >  1:1115491/1‑90 (MQ=255)          aTTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGtt                                                                          <  2:937304/90‑1 (MQ=255)            ttAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTg                                                                        >  1:333787/1‑90 (MQ=255)             tagtagACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGc                                                                       >  2:486967/1‑90 (MQ=255)              agtagACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGcc                                                                      <  1:538346/90‑1 (MQ=255)               gtagACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGccc                                                                     >  2:264613/1‑90 (MQ=255)                  gACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGt                                                                  >  2:813234/1‑90 (MQ=255)                  gACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGt                                                                  >  2:230768/1‑90 (MQ=255)                   aCAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGtt                                                                 <  2:32690/90‑1 (MQ=255)                   aCAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGtt                                                                 <  2:112102/90‑1 (MQ=255)                     aGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTgg                                                               >  1:1132274/1‑90 (MQ=255)                      gcgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCCCAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGa                                                              <  1:525230/90‑1 (MQ=255)                      gcgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGa                                                              >  1:489930/1‑90 (MQ=255)                        gcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTc                                                                               >  1:998377/1‑71 (MQ=255)                        gcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTc                                                                               <  2:998377/71‑1 (MQ=255)                        gcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATc                                                            <  2:7680/90‑1 (MQ=255)                         cACCCATTGCGTCGAATTCTCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAccac                                                                                     >  1:206734/1‑64 (MQ=255)                         cACCCATTGCGTCGAATTCTCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAccac                                                                                     <  2:206734/64‑1 (MQ=255)                              aTTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGctct                                                      >  1:458636/1‑90 (MQ=255)                                tGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTAc                                                    <  1:7012/90‑1 (MQ=255)                                     cGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTc                                                                               <  1:104801/58‑1 (MQ=255)                                     cGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTc                                                                               >  2:104801/1‑58 (MQ=255)                                     cGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGt                                               >  2:843256/1‑90 (MQ=255)                                      gAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                              <  1:884164/90‑1 (MQ=255)                                      gAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                              <  1:919358/90‑1 (MQ=255)                                      gAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                              <  2:814757/90‑1 (MQ=255)                                      gAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                              <  1:230768/90‑1 (MQ=255)                                      gAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                              <  1:704576/90‑1 (MQ=255)                                            gCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCTTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGAt                                        <  1:518558/90‑1 (MQ=255)                                              aaGCCTTTCCCCAGCACTGCTCTTCGCCGCCGCCAGCAACCACGCTGTCCTGTTTGCCCAGGTGGATCTGCTCTACTGCGTTACCGATAc                                      <  1:127030/90‑1 (MQ=255)                                              aaGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATAc                                      <  1:813234/90‑1 (MQ=255)                                              aaGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATAc                                      <  2:863429/90‑1 (MQ=255)                                              aaGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATAc                                      <  2:319558/90‑1 (MQ=255)                                              aaGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATAc                                      <  2:562789/90‑1 (MQ=255)                                                gCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAg                                    <  2:881931/90‑1 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGcac                    <  2:489930/90‑1 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGcac                    >  1:139396/1‑90 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGcac                    >  1:792392/1‑90 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGcac                    >  1:299748/1‑90 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGcac                    >  1:367460/1‑90 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGc                      <  2:1028196/88‑1 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGc                      >  1:1028196/1‑88 (MQ=255)                                                                       gccgccgccAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGa             <  2:833658/90‑1 (MQ=255)                                                                       gccgccgccAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGa             <  1:1213154/90‑1 (MQ=255)                                                                        ccgccgccAGCAACCACGATGTCCTGTTTGCCCAGtt                                                                 <  1:141668/37‑1 (MQ=38)                                                                        ccgccgccAGCAACCACGATGTCCTGTTTGCCCAGtt                                                                 >  2:141668/1‑37 (MQ=38)                                                                        ccgccgccAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGAg            >  2:263004/1‑90 (MQ=255)                                                                          gccgccAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                              <  2:399516/54‑1 (MQ=255)                                                                          gccgccAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                              >  1:399516/1‑54 (MQ=255)                                                                            cgccAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGAGCTGa        <  2:457878/90‑1 (MQ=255)                                                                                  cAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGAGCTGATGGagt  <  1:622010/90‑1 (MQ=255)                                                                                     |                                                                                       TGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGAGCTGATGGAGT  >  NZ_CP009273/2434445‑2434615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑