Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,753,169	T→C	S276P (TCA→CCA)	yfjI →	YfjI family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,753,169	0	T	C	100.0%	142.1 / NA	41	S276P (TCA→CCA)	yfjI	YfjI family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/17);  total (24/17)

AAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTTCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATGAAAATAATC  >  NZ_CP009273/2753084‑2753250                                                                                      |                                                                                  aaaGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCaaa                                                                               >  2:512118/1‑90 (MQ=255)       gattggattTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACa                                                                         >  1:779458/1‑90 (MQ=255)       gattggattTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACa                                                                         >  1:42459/1‑90 (MQ=255)             attTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGcc                                                                   <  2:218368/90‑1 (MQ=255)              ttttttGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCg                                                                  >  2:189729/1‑90 (MQ=255)              ttttttGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCg                                                                  <  1:1073870/90‑1 (MQ=255)              ttttttGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCg                                                                  <  1:296046/90‑1 (MQ=255)                          tGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCaa                                                      <  2:474149/90‑1 (MQ=255)                          tGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCaa                                                      <  2:26271/90‑1 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTGACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                             >  1:704943/1‑90 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCGACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                             >  2:874311/1‑90 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAATCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                             >  1:310614/1‑90 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCaa                                                      <  2:715769/81‑1 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCaa                                                      >  1:715769/1‑81 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                             >  1:1045067/1‑90 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                             >  1:446012/1‑90 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                             >  2:761820/1‑90 (MQ=255)                                   tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                             >  1:1226999/1‑90 (MQ=255)                                     ccAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGa                                           >  2:1087924/1‑90 (MQ=255)                                      cAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGaa                                          <  2:279753/90‑1 (MQ=255)                                      cAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGaa                                          <  2:1012849/90‑1 (MQ=255)                                      cAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGaa                                          <  1:883261/90‑1 (MQ=255)                                      cAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGaa                                          <  1:634059/90‑1 (MQ=255)                                       aGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGaaa                                         >  1:464048/1‑90 (MQ=255)                                        gCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTc                                                           <  2:1023564/71‑1 (MQ=255)                                        gCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTc                                                           >  1:1023564/1‑71 (MQ=255)                                        gCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAAt                                        <  1:229556/90‑1 (MQ=255)                                         ccTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAAtt                                       >  2:927933/1‑90 (MQ=255)                                            gCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCa                                                       >  1:490585/1‑71 (MQ=255)                                            gCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCa                                                       <  2:490585/71‑1 (MQ=255)                                                cAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATg                                <  1:415972/90‑1 (MQ=255)                                                  acacAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATgag                              >  2:1195656/1‑90 (MQ=255)                                                  acacAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATgag                              >  2:719195/1‑90 (MQ=255)                                                  acacAAGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATgag                              >  2:811313/1‑90 (MQ=255)                                                       aGGTAACAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGcat                         >  2:138584/1‑90 (MQ=255)                                                            aCAGAAAAATTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTa                    >  2:432275/1‑90 (MQ=255)                                                                 aaaaTTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGc                           <  1:805962/78‑1 (MQ=255)                                                                 aaaaTTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGc                           >  2:805962/1‑78 (MQ=255)                                                                     tttCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATg           <  1:138584/90‑1 (MQ=255)                                                                     tttCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATg           <  2:103004/90‑1 (MQ=255)                                                                              cAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATGAAAATAATc  >  1:1152218/1‑90 (MQ=255)                                                                                      |                                                                                  AAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTTCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATGAAAATAATC  >  NZ_CP009273/2753084‑2753250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑