Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,801,112	A→C	T208P (ACC→CCC)	proX →	glycine betaine/L‑proline ABC transporter substrate‑binding protein ProX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,801,112	0	A	C	100.0%	132.9 / NA	39	T208P (ACC→CCC)	proX	glycine betaine/L‑proline ABC transporter substrate‑binding protein ProX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/19);  total (20/19)

CGTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCA  >  NZ_CP009273/2801027‑2801189                                                                                      |                                                                              cGTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTg                                                                           <  1:979361/90‑1 (MQ=255) cGTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTg                                                                           <  1:445423/90‑1 (MQ=255)  gTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTgg                                                                          >  2:184437/1‑90 (MQ=255)  gTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTgg                                                                          >  1:421072/1‑90 (MQ=255)  gTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTg                                                                           <  2:199420/89‑1 (MQ=255)  gTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTg                                                                           >  1:199420/1‑89 (MQ=255)     aCGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACg                                                                       <  2:1021556/90‑1 (MQ=255)     aCGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACg                                                                       <  1:509518/90‑1 (MQ=255)      cGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGTAAACCGGTGTTTTATTACCCCTGGACGc                                                                      <  2:921115/90‑1 (MQ=255)      cGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGc                                                                      <  1:1072281/90‑1 (MQ=255)      cGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGc                                                                      <  2:281564/90‑1 (MQ=255)      cGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGc                                                                      <  1:8095/90‑1 (MQ=255)      cGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGc                                                                      <  2:350215/90‑1 (MQ=255)      cGCATAATCAGGGGAACTACGAAGCGATGATGGCCGACACCATCAGTGGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGc                                                                      <  1:983422/90‑1 (MQ=255)            aTCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACt                                                                <  1:1009049/90‑1 (MQ=255)                ggggAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGt                                                            >  2:217236/1‑90 (MQ=255)                     aCTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTa                                                       >  2:722468/1‑90 (MQ=255)                        aCGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACg                                                    >  1:1167724/1‑90 (MQ=255)                             gCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTg                                               >  2:755219/1‑90 (MQ=255)                             gCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTg                                               >  2:771319/1‑90 (MQ=255)                                  gatgGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGcc                                          >  1:1140156/1‑90 (MQ=255)                                     ggCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGGGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCggg                                       >  1:1155555/1‑90 (MQ=255)                                                   gTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCt                         <  1:948752/90‑1 (MQ=255)                                                     cGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTgg                       >  1:959073/1‑90 (MQ=255)                                                       cTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGtt                     >  2:321378/1‑90 (MQ=255)                                                            aaGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAgg                <  1:1190061/90‑1 (MQ=255)                                                               agGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACTAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGc             <  2:1122994/90‑1 (MQ=255)                                                               agGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGc             <  1:708558/90‑1 (MQ=255)                                                                gggCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGcc            >  2:1191062/1‑90 (MQ=255)                                                                 ggCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGc             <  1:1069641/88‑1 (MQ=255)                                                                 ggCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGc             >  2:1069641/1‑88 (MQ=255)                                                                 ggCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCg           >  2:571857/1‑90 (MQ=255)                                                                 ggCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCg           <  2:667995/90‑1 (MQ=255)                                                                     aaCCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTtct       >  2:633217/1‑90 (MQ=255)                                                                          gtgtTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGc                                           <  1:86481/49‑1 (MQ=255)                                                                          gtgtTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGc                                           >  2:86481/1‑49 (MQ=255)                                                                          gtgtTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCa  >  1:966097/1‑90 (MQ=255)                                                                          gtgtTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCa  >  1:851556/1‑90 (MQ=255)                                                                          gtgtTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCa  >  1:405653/1‑90 (MQ=255)                                                                                      |                                                                              CGTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCA  >  NZ_CP009273/2801027‑2801189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑