Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,819,095	T→C	intergenic (‑160/‑96)	mltB ← / → srlA	lytic murein transglycosylase B/PTS glucitol/sorbitol transporter subunit IIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,819,095	0	T	C	100.0%	88.6 / NA	27	intergenic (‑160/‑96)	mltB/srlA	lytic murein transglycosylase B/PTS glucitol/sorbitol transporter subunit IIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/13);  total (14/13)

GACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAG  >  NZ_CP009273/2819024‑2819181                                                                        |                                                                                       gACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATa                                                                      >  2:1005499/1‑90 (MQ=255)   cTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAac                                                                    <  2:135208/90‑1 (MQ=255)   cTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAac                                                                    <  2:254799/90‑1 (MQ=255)     ttACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAacac                                                                  >  1:12299/1‑90 (MQ=255)      tACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACt                                                                 >  1:1210435/1‑90 (MQ=255)           aactcaactcATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTaa                                                            >  1:626309/1‑90 (MQ=255)           aactcaactcATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTaa                                                            >  1:104447/1‑90 (MQ=255)               caactcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCt                                                        >  2:1225591/1‑90 (MQ=255)               caactcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCt                                                        >  1:760198/1‑90 (MQ=255)                 actcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCttt                                                      >  1:666416/1‑90 (MQ=255)                 actcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCttt                                                      >  2:1040652/1‑90 (MQ=255)                      ttCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATc                                                 <  2:203189/90‑1 (MQ=255)                      ttCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATc                                                 <  2:12299/90‑1 (MQ=255)                      ttCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATc                                                 <  1:703876/90‑1 (MQ=255)                      ttCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATc                                                 >  2:635861/1‑90 (MQ=255)                         cccTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTga                                              >  1:992207/1‑90 (MQ=255)                          ccTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTgat                                             <  1:294834/90‑1 (MQ=255)                          ccTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTgat                                             <  2:721727/90‑1 (MQ=255)                          ccTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTCCATTCTGCGATCAAAATAACACTTTTAAATCTTTCAATCTgat                                             <  1:838069/90‑1 (MQ=255)                                       ttATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGc                                >  2:96160/1‑90 (MQ=255)                                       ttATTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGc                                >  1:998560/1‑90 (MQ=255)                                         aTTCAAACTTTCAAATTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGcct                              >  2:747610/1‑89 (MQ=255)                                                      aaTTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATaaaa                 <  2:1093582/90‑1 (MQ=255)                                                      aaTTAAAATATTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATaaaa                 <  2:544791/90‑1 (MQ=255)                                                           aaaTACTTATCTTCCGGTTTGCCAACAAAATAACACAAATAAATCTTTCACCCTCACTAGATTAGGTTGCCGTTTGGTaacaaaacaaaa            <  1:1112974/90‑3 (MQ=255)                                                              tatTTATCTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAAt         <  1:604034/90‑1 (MQ=255)                                                                     cTTCCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAg  <  1:1102391/90‑1 (MQ=255)                                                                        |                                                                                       GACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAG  >  NZ_CP009273/2819024‑2819181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑