Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,833,262	T→G	V127G (GTC→GGC)	ascF →	PTS cellobiose/arbutin/salicin transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,833,262	0	T	G	100.0%	165.7 / NA	49	V127G (GTC→GGC)	ascF	PTS cellobiose/arbutin/salicin transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/23);  total (26/23)

CGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGAC  >  NZ_CP009273/2833175‑2833347                                                                                        |                                                                                      cGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCa                                                                                     >  2:141567/1‑90 (MQ=255)     aCGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAAct                                                                                 >  1:1222135/1‑90 (MQ=255)       gTCGCATTGGTGCGGGGACCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAActgc                                                                               <  2:149942/90‑1 (MQ=255)        tCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAActgct                                                                              <  2:1193846/90‑1 (MQ=255)             ttGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCaa                                                                         >  1:1054639/1‑90 (MQ=255)               ggTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATg                                                                       <  1:141567/90‑1 (MQ=255)                    ggggATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCt                                                                  >  1:517514/1‑90 (MQ=255)                    ggggATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCt                                                                  >  2:253184/1‑90 (MQ=255)                         tCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCgaga                                                             >  2:918328/1‑90 (MQ=255)                         tCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCgaga                                                             >  2:248760/1‑90 (MQ=255)                          ccTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGAt                                                            >  2:790564/1‑90 (MQ=255)                           cTCGATGCGCTGATCGGCACCATGTCACCGCTGATTCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATg                                                           <  2:336844/90‑1 (MQ=255)                                 gcgcTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAgcggc                                                     >  1:1067811/1‑90 (MQ=255)                                     tGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGc                                                 <  1:605273/90‑1 (MQ=255)                                     tGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGc                                                 <  1:63795/90‑1 (MQ=255)                                     tGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGc                                                 <  1:501811/90‑1 (MQ=255)                                      gATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTTCTGGCAATGATCCTCGAGATGAGCGGCGTGCt                                                >  1:794607/1‑90 (MQ=255)                                       aTCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTg                                               <  1:377948/90‑1 (MQ=255)                                              ccATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAg                                        >  2:898949/1‑90 (MQ=255)                                                aTGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGGTCCTCGAGATGAGCGGCGGGCGGGCAAAAGGa                                      >  1:625381/1‑90 (MQ=255)                                                aTGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGa                                      >  2:424589/1‑90 (MQ=255)                                                aTGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGa                                      >  2:186206/1‑90 (MQ=255)                                                aTGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCCCGAGATGAGCGGGGTGCTGACAAAAGGa                                      >  1:1164737/1‑90 (MQ=255)                                                    cACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGc                                  <  1:408562/90‑1 (MQ=255)                                                     aCCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAGGGATCGcc                                 <  1:248760/90‑1 (MQ=255)                                                     aCCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGcc                                 <  1:1206386/90‑1 (MQ=255)                                                     aCCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGcc                                 <  2:710515/90‑1 (MQ=255)                                                        gCTGATCCCGGCGATTATCGGCGGATCGATGGGCaa                                                                                    >  1:671300/1‑36 (MQ=255)                                                        gCTGATCCCGGCGATTATCGGCGGATCGATGGGCaa                                                                                    <  2:671300/36‑1 (MQ=37)                                                        gCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGAc                              >  2:443600/1‑90 (MQ=255)                                                        gCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGAc                              >  1:1057646/1‑90 (MQ=255)                                                        gCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGAc                              >  1:948100/1‑90 (MQ=255)                                                         cTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGAcc                             <  2:948100/90‑1 (MQ=255)                                                           gATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCtt                           >  1:257861/1‑90 (MQ=255)                                                           gATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCtt                           >  1:112888/1‑90 (MQ=255)                                                           gATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCtt                           <  1:116265/90‑1 (MQ=255)                                                           gATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCtt                           >  2:423389/1‑90 (MQ=255)                                                              cccGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAAc                        <  1:424589/90‑1 (MQ=255)                                                                cGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCCATGGTCCCCGCGATGGGAGGCGTGCTGACAAAAGGATCGCCGACCTTAACCa                      >  1:885634/1‑90 (MQ=255)                                                                cGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCa                      >  1:1123422/1‑90 (MQ=255)                                                                cGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCa                      >  2:919741/1‑90 (MQ=255)                                                                   cGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTc                   <  1:1193803/90‑1 (MQ=255)                                                                   cGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTc                   <  1:823571/90‑1 (MQ=255)                                                                      ttATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGa                <  1:325586/90‑1 (MQ=255)                                                                      ttATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGa                <  2:1123422/90‑1 (MQ=255)                                                                                    aTGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGAc  <  1:405052/90‑1 (MQ=255)                                                                                    aTGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGAc  >  2:1124412/1‑90 (MQ=255)                                                                                    aTGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGAc  <  2:568532/90‑1 (MQ=255)                                                                                    aTGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGAc  <  1:253184/90‑1 (MQ=255)                                                                                        |                                                                                      CGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGAC  >  NZ_CP009273/2833175‑2833347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑