Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,011,010	A→G	E531G (GAA→GGA)	ygfK →	putative selenate reductase subunit YgfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,011,010	0	A	G	100.0%	162.5 / NA	47	E531G (GAA→GGA)	ygfK	putative selenate reductase subunit YgfK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/22);  total (25/22)

TTTGCGATCACCAGTGCC‑AATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATT  >  NZ_CP009273/3010923‑3011086                                                                                         |                                                                             tttGCGAT‑ACCAGCGCCAAACACAACTGTCCCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGgaa                                                                            <  2:85369/90‑1 (MQ=255)        tccccAGTGCC‑AATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTg                                                                     <  1:606606/87‑1 (MQ=255)           ccAGTCCA‑AAAAAAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGa                                                                  <  1:634543/90‑1 (MQ=255)            cAGTGCA‑AAAAAAACTGTCCCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGAt                                                                 <  2:711002/90‑1 (MQ=255)            cAGTCCA‑AATACAACTGTCCCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGAt                                                                 <  2:576379/90‑1 (MQ=255)                 cc‑AATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAata                                                            <  2:120854/90‑1 (MQ=255)                         aaCTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGgaaaa                                                                          >  2:868962/1‑69 (MQ=255)                         aaCTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGgaaaa                                                                          <  1:868962/69‑1 (MQ=255)                         aaCTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCaa                                                     >  1:951696/1‑90 (MQ=255)                         aaCTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCaa                                                     >  2:910438/1‑90 (MQ=255)                         aaCTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGCTGGGAGGAATATAAGCaa                                                     >  1:449727/1‑90 (MQ=255)                          aCTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAAc                                                    >  1:345494/1‑90 (MQ=255)                                  cGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGcac                                            >  2:825057/1‑90 (MQ=255)                                  cGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGcac                                            >  1:802468/1‑90 (MQ=255)                                  cGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGcac                                            >  1:26686/1‑90 (MQ=255)                                       ggATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAAcc                                       >  2:81711/1‑90 (MQ=255)                                           tACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGcc                                   <  2:961863/90‑1 (MQ=255)                                            aCGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCg                                  <  2:133299/90‑1 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGgaaaa                                                                          >  1:60962/1‑44 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGgaaaa                                                                          <  2:60962/44‑1 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGAt                                                                 >  2:222055/1‑53 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGAt                                                                 <  1:222055/53‑1 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAata                                                            >  2:1198558/1‑58 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAata                                                            <  1:1198558/58‑1 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAAc                                        <  1:563982/78‑1 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAAc                                        >  2:563982/1‑78 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTc                              >  1:949364/1‑88 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTc                              <  2:949364/88‑1 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCt                             >  2:346476/1‑89 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCt                             <  1:346476/89‑1 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTg                            >  1:944698/1‑90 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTg                            >  1:827221/1‑90 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTg                            >  2:272623/1‑90 (MQ=255)                                                  gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTg                            >  1:382802/1‑90 (MQ=255)                                                    gcgcTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGt                          >  2:1109813/1‑90 (MQ=255)                                                      gcTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTc                        >  1:748930/1‑90 (MQ=255)                                                      gcTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTc                        <  2:183174/90‑1 (MQ=255)                                                       cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCa                       <  2:1151697/90‑1 (MQ=255)                                                        tGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCAc                      <  1:66777/90‑1 (MQ=255)                                                         gAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTg                                                                     <  2:305477/42‑1 (MQ=255)                                                         gAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTg                                                                     >  1:305477/1‑42 (MQ=255)                                                          aaTATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGc                    <  2:116657/90‑1 (MQ=255)                                                          aaTATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGc                    <  2:345494/90‑1 (MQ=255)                                                            tatCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCa                  >  1:1143943/1‑90 (MQ=255)                                                              tCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATc                >  1:702163/1‑90 (MQ=255)                                                              tCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATc                >  1:608037/1‑90 (MQ=255)                                                                  cgACCTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGt            <  2:586948/90‑1 (MQ=255)                                                                   gAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGtt           >  2:435582/1‑90 (MQ=255)                                                                   gAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGtt           >  1:291655/1‑90 (MQ=255)                                                                    aaCTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTg          <  2:827221/90‑1 (MQ=255)                                                                       tGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCg       <  2:438414/90‑1 (MQ=255)                                                                            aaaGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGAtt  <  2:988467/90‑1 (MQ=255)                                                                                         |                                                                             TTTGCGATCACCAGTGCC‑AATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATT  >  NZ_CP009273/3010923‑3011086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑