Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,179,271	G→A	A167T (GCC→ACC)	ygiL →	fimbrial‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,179,271	0	G	A	100.0%	151.7 / NA	44	A167T (GCC→ACC)	ygiL	fimbrial‑like protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/22);  total (22/22)

ACTCCGGTAGTAATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACA  >  NZ_CP009273/3179187‑3179353                                                                                     |                                                                                   aCTCCGGTAGTAATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATaccacc                                                                               >  2:190326/1‑90 (MQ=255)  cTCCGGTAGTAATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCAccc                                                                              >  2:947678/1‑90 (MQ=255)  cTCCGGTAGTAATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCAccc                                                                              >  1:168532/1‑90 (MQ=255)  cTCCGGTAGTAATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCAccc                                                                              >  2:203018/1‑90 (MQ=255)  cTCCGGTAGTAATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCAccc                                                                              >  2:186500/1‑90 (MQ=255)   tCCGGTAGTAATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCAcccc                                                                             >  1:72005/1‑90 (MQ=255)    ccGGTAGTAATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCg                                                                            <  2:180397/90‑1 (MQ=255)        tagtaATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCa                                                                        <  1:842043/90‑1 (MQ=255)        tagtaATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCa                                                                        <  1:346551/90‑1 (MQ=255)            aaTTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGt                                                                    >  2:619930/1‑90 (MQ=255)            aaTTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGt                                                                    >  2:486150/1‑90 (MQ=255)              ttACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGttt                                                                  <  1:1115122/90‑1 (MQ=255)              ttACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGttt                                                                  >  1:1074681/1‑90 (MQ=255)                aCGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTAc                                                                <  2:1074681/90‑1 (MQ=255)                   ttCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCt                                                             >  2:496867/1‑90 (MQ=255)                    tCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTc                                                            <  1:962523/90‑1 (MQ=255)                        caaTACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGc                                                        <  1:486150/90‑1 (MQ=255)                        caaTACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGc                                                        <  1:479586/90‑1 (MQ=255)                          acaCCAACAGCCATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCACGCTg                                                      <  1:629181/88‑1 (MQ=255)                          aTACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATACCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTg                                                      <  1:718537/90‑1 (MQ=255)                               aaCAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTAc                                                 >  2:1145551/1‑90 (MQ=255)                                      aTCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTg                                          >  2:312053/1‑90 (MQ=255)                                           gAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTAc                                     >  2:355504/1‑90 (MQ=255)                                                   tttCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATc                             <  1:76048/90‑1 (MQ=255)                                                    ttCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCg                            <  1:496867/90‑1 (MQ=255)                                                          gCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGAt                                            >  1:484674/1‑68 (MQ=255)                                                          gCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGAt                                            <  2:484674/68‑1 (MQ=255)                                                          gCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTg                      >  1:743202/1‑90 (MQ=255)                                                            tCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTg                                                      <  2:910247/56‑1 (MQ=255)                                                            tCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTg                                                      >  1:910247/1‑56 (MQ=255)                                                            tCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGc                    >  1:916399/1‑90 (MQ=255)                                                                    aaTCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCg            >  2:1238394/1‑90 (MQ=255)                                                                    aaTCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCg            >  1:808369/1‑90 (MQ=255)                                                                       ccccAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTg                                                      >  1:382137/1‑45 (MQ=255)                                                                       ccccAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTg                                                      <  2:382137/45‑1 (MQ=255)                                                                       ccccAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGccc         >  1:287612/1‑90 (MQ=255)                                                                       ccccAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGccc         >  2:121849/1‑90 (MQ=255)                                                                       ccccAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGccc         <  1:926384/90‑1 (MQ=255)                                                                       ccccAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGccc         >  1:551240/1‑90 (MQ=255)                                                                            aGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCca    <  1:619930/90‑1 (MQ=255)                                                                            aGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCca    <  1:196173/90‑1 (MQ=255)                                                                            aGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCca    <  1:652314/90‑1 (MQ=255)                                                                            aGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCca    <  1:788644/90‑1 (MQ=255)                                                                             gCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCcac   <  2:430745/90‑1 (MQ=255)                                                                              cAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCcaca  <  1:388174/90‑1 (MQ=255)                                                                                     |                                                                                   ACTCCGGTAGTAATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACA  >  NZ_CP009273/3179187‑3179353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑