Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,318,468	A→C	A609A (GCT→GCG)	ftsH ←	ATP‑dependent zinc metalloprotease FtsH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,318,468	0	A	C	100.0%	172.9 / NA	51	A609A (GCT→GCG)	ftsH	ATP‑dependent zinc metalloprotease FtsH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/29);  total (22/29)

ATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGAAGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGATAGTCTCAT  >  NZ_CP009273/3318385‑3318553                                                                                    |                                                                                      aTGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCt                                                                                 <  2:48708/90‑1 (MQ=255) aTGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCt                                                                                 <  1:332640/90‑1 (MQ=255)   ggTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTgg                                                                               >  1:214005/1‑90 (MQ=255)     tgtTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGtt                                                                             <  2:273839/90‑1 (MQ=255)      gtTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGttc                                                                            <  1:1209438/90‑1 (MQ=255)      gtTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGttc                                                                            <  1:847524/90‑1 (MQ=255)        tACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGttctt                                                                          >  1:274761/1‑90 (MQ=255)             gggTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAg                                                                     >  1:331238/1‑90 (MQ=255)              ggTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGc                                                                    >  2:982300/1‑90 (MQ=255)              ggTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGc                                                                    >  2:972091/1‑90 (MQ=255)              ggTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGc                                                                    >  1:1050836/1‑90 (MQ=255)              ggTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGc                                                                    >  2:605552/1‑90 (MQ=255)              ggTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGc                                                                    >  2:1130686/1‑90 (MQ=255)              ggTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGCCGCCAGAATAGTTAGACGCGCCTGGATCTTCCCAGc                                                                    >  1:550601/1‑90 (MQ=255)               gTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGcc                                                                   <  1:313507/90‑1 (MQ=255)                  cGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGc                                                                >  1:79294/1‑90 (MQ=255)                   ggCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCt                                                               >  1:508353/1‑90 (MQ=255)                    gCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTg                                                              >  1:589562/1‑90 (MQ=255)                      gTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGc                                                            <  1:798210/90‑1 (MQ=255)                      gTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGc                                                            <  2:779683/90‑1 (MQ=255)                      gTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGc                                                            <  2:915510/90‑1 (MQ=255)                      gTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGc                                                            <  1:518540/90‑1 (MQ=255)                         cgcgGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGa                                                         <  1:396464/90‑1 (MQ=255)                            ggTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGt                                                      <  1:189612/90‑1 (MQ=255)                                  tcaACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATcg                                                >  1:779216/1‑90 (MQ=255)                                  tcaACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATcg                                                >  2:29145/1‑90 (MQ=255)                                       cGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACg                                           <  2:1050836/90‑1 (MQ=255)                                       cGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACg                                           <  2:1077649/90‑1 (MQ=255)                                       cGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACg                                           <  2:308949/90‑1 (MQ=255)                                       cGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATCGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACg                                           >  1:198609/1‑90 (MQ=255)                                        ggACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGt                                          <  1:588591/90‑1 (MQ=255)                                             aggagCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCAt                                     >  2:738802/1‑90 (MQ=255)                                                agCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAg                                  <  2:66537/90‑1 (MQ=255)                                                     ttGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGtcat                             <  2:938782/90‑1 (MQ=255)                                                     ttGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGtcat                             <  1:436933/90‑1 (MQ=255)                                                          cTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATc                        <  1:825609/90‑1 (MQ=255)                                                           taacATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCt                       >  1:1062716/4‑90 (MQ=255)                                                           tACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCa                                      >  2:57641/1‑75 (MQ=255)                                                           tACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCa                                      <  1:57641/75‑1 (MQ=255)                                                           tACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCt                       >  1:128998/1‑90 (MQ=255)                                                             ccATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGc                     <  2:504699/90‑1 (MQ=255)                                                              cATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCg                    <  1:339809/90‑1 (MQ=255)                                                                  gTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGc                >  1:1210725/1‑90 (MQ=255)                                                                    cGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGtc                               <  2:126434/73‑1 (MQ=255)                                                                    cGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGtc                               >  1:126434/1‑73 (MQ=255)                                                                        aGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGAt          <  2:769239/90‑1 (MQ=255)                                                                        aGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGAt          <  1:463156/90‑1 (MQ=255)                                                                         gAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGATa         >  2:184204/1‑90 (MQ=255)                                                                                tAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGATAGTCTCat  <  2:169019/90‑1 (MQ=255)                                                                                tAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGATAGTCTCat  <  2:79294/90‑1 (MQ=255)                                                                                tAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGATAGTCTCat  <  2:867737/90‑1 (MQ=255)                                                                                    |                                                                                      ATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGAAGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGATAGTCTCAT  >  NZ_CP009273/3318385‑3318553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑