Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,084,653	A→G	G358G (GGT→GGC)	frvB ←	PTS fructose‑like transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,084,653	0	A	G	100.0%	97.0 / NA	32	G358G (GGT→GGC)	frvB	PTS fructose‑like transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/11);  total (21/11)

GTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGA  >  NZ_CP009273/4084573‑4084738                                                                                 |                                                                                      gtgGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCcgcg                                                                              >  2:622033/1‑90 (MQ=255)     cACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACgg                                                                          >  1:24494/1‑90 (MQ=255)       ccGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGAAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggc                                                                        <  1:314737/90‑1 (MQ=255)         gACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggcgg                                                                      >  2:656676/1‑90 (MQ=255)             acaATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATc                                                                  <  2:744693/90‑1 (MQ=255)                aTGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAgc                                                               <  1:1219616/90‑1 (MQ=255)                   gCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGcgcg                                                            >  2:1104588/1‑90 (MQ=255)                     actactGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGAc                                                          >  2:792099/1‑90 (MQ=255)                        actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                       >  2:1179302/1‑90 (MQ=255)                        actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                       >  2:907358/1‑90 (MQ=255)                        actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                       >  2:283945/1‑90 (MQ=255)                          tGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGc                                                     <  2:922092/90‑1 (MQ=255)                           ggCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGcc                                                    >  1:308619/1‑90 (MQ=255)                            gCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcg                                                   >  1:651068/1‑90 (MQ=255)                              aGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcgac                                                 >  2:1106706/1‑90 (MQ=255)                              aGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcgac                                                 <  2:62807/90‑1 (MQ=255)                                         ccTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAcc                                      <  1:202986/90‑1 (MQ=255)                                          cTGTGGGGTAAACAGTTTGGGCGCAATGACCGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCa                                     <  2:682130/90‑1 (MQ=255)                                          cTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCa                                     <  2:665095/90‑1 (MQ=255)                                           tGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAg                                    >  1:787376/1‑90 (MQ=255)                                             tCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTc                                  >  2:138/1‑90 (MQ=255)                                                        gTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGtt                       <  1:842488/90‑1 (MQ=255)                                                        gTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGtt                       >  2:416337/1‑90 (MQ=255)                                                          ttGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcgacg                                                >  1:592815/1‑63 (MQ=255)                                                          ttGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGtttt                     >  1:794093/1‑90 (MQ=255)                                                          ttGGGCGCAATGAACGCCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcgacg                                                <  2:592815/63‑1 (MQ=255)                                                                  aaTGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagcag             >  2:706366/1‑90 (MQ=255)                                                                  aaTGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagcag             >  1:402477/1‑90 (MQ=255)                                                                  aaTGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagcag             >  1:1033522/1‑90 (MQ=255)                                                                  aaTGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagcag             >  2:1106379/1‑90 (MQ=255)                                                                          tCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGc     >  1:428781/1‑90 (MQ=255)                                                                             cAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCaga  <  2:787376/90‑1 (MQ=255)                                                                                 |                                                                                      GTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGA  >  NZ_CP009273/4084573‑4084738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑