Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,153,816	T→C	S84P (TCA→CCA)	btuB →	TonB‑dependent vitamin B12 receptor BtuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,153,816	0	T	C	100.0%	147.1 / NA	45	S84P (TCA→CCA)	btuB	TonB‑dependent vitamin B12 receptor BtuB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/22);  total (23/22)

CTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGG  >  NZ_CP009273/4153734‑4153901                                                                                   |                                                                                      cTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGGTCAGGGCAGCTCCCACCTAt                                                                                >  1:761470/1‑90 (MQ=255)      aGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTTAGGGCAGCTCCCCTCTAtttttt                                                                           >  1:119932/1‑89 (MQ=255)       gTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTAt                                                                          >  2:66749/1‑90 (MQ=255)        tCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTAtttttttt                                                                         >  1:1067799/1‑90 (MQ=255)         cGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTc                                                                        <  1:1699/90‑1 (MQ=255)           aCCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTcgc                                                                      <  2:900243/90‑1 (MQ=255)           aCCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTcgc                                                                      <  2:767994/90‑1 (MQ=255)           aCCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTcgc                                                                      <  1:794494/90‑1 (MQ=255)           aCCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTcgc                                                                      <  1:264723/90‑1 (MQ=255)             cTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCgg                                                                    <  1:64040/90‑1 (MQ=255)                 gTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTTTTCGCGGGACa                                                                >  2:484953/1‑90 (MQ=255)                  tCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTGTTTTTATTCGCGGTACaa                                                               <  1:1134922/90‑1 (MQ=255)                  tCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACaa                                                               <  1:541836/90‑1 (MQ=255)                    aaTGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAAt                                                             <  1:3778/90‑1 (MQ=255)                     atgatgTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATg                                                            >  2:75030/1‑90 (MQ=255)                       gatgTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGcc                                                          <  2:1182363/90‑1 (MQ=255)                       gatgTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGcc                                                          <  1:562269/90‑1 (MQ=255)                       gatgTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGcc                                                          <  1:619452/90‑1 (MQ=255)                          gtgCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTTAGGGCAGCTCCCATCTATTTTTATTTGCGGGACAAAAGCCAGt                                                       >  1:503973/1‑90 (MQ=255)                          gtgCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTTTTCGCGGTACAAAAGCCCGt                                                       >  2:1229799/1‑90 (MQ=255)                           tgctgcGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGGACAAATGCCAGTc                                                      >  2:915234/1‑90 (MQ=255)                           tgctgcGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGGCAGCTCCCATCTATTTTTTTTCGCGGTACAAATGCCAGTc                                                      >  2:511179/1‑90 (MQ=255)                            gctgcGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCa                                                     <  1:718854/90‑1 (MQ=255)                            gctgcGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCa                                                     <  1:1121524/90‑1 (MQ=255)                               gcgcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGGCAGCTCCCATCTATTTTTTTTTGCGGGACAAAAGCCAGTCAtgt                                                  >  1:369287/1‑90 (MQ=255)                                 gcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATgtgt                                                <  1:930528/90‑1 (MQ=255)                                    gTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTgg                                             >  2:477753/1‑90 (MQ=255)                                    gTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGGCAGCTCCCCTCTATTTTTTTTTGCGGGACAAAAGCCAGTCATGTGTTgg                                             >  1:105647/1‑90 (MQ=255)                                              cGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTTTTCGCGGGACCAATGCCAGTCATGTGTTGGGGTTTATTGa                                   >  2:1159820/1‑90 (MQ=255)                                              cGTCGATATCACCCAAAACGGCGGTTCAGGGCAGCTCCCCTCTATTTTTTTTTGCGGGACAAAAGCCCGGCATGTGTTGGTGTTTATTGa                                   >  1:678402/1‑90 (MQ=255)                                              cGTCGATATCACCCAAAACGGCGGTTCAGGGCAGCTCCCATCTATTTTTTTTTGCGGGACAAAAGCCAGTCATGTGTTTGGGTTAATTGa                                   >  1:243758/1‑90 (MQ=255)                                              cGTCGATATCACCCAAAACGGCGGTTCACGGCCGCTCCCCTCCATTTTTTTTTGCGGGACAAAAGCCAGTCCTGGGTTTGTGTTTATTGa                                   >  1:633700/1‑90 (MQ=255)                                               gTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGAt                                  <  2:1010686/90‑1 (MQ=255)                                               gTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGAt                                  <  1:158047/90‑1 (MQ=255)                                               gTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCAGCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGAt                                  <  2:909181/90‑1 (MQ=255)                                                  gATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGGGTTAATTGATGGc                               >  2:784399/1‑90 (MQ=255)                                                   atatCACCCAAAACGGCGGTTCAGGTCAGCTCCCCTCTATTTTTTTTTGCGGGACAAATGCCAGTCATGTGTTGGGGGTAATTTATGGCg                              >  1:376486/1‑90 (MQ=255)                                                             aaaCGGCGGTTCAGGTCAGCTCCCATCTATTTTTTTTCGCGGTACAAAAGCCCGTCATGTGTTGGGGTTTATTTTTGGCGTACGCCTGaa                    >  1:632182/1‑90 (MQ=255)                                                              aaCGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAAt                   <  2:214989/90‑1 (MQ=255)                                                                   cggTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGc              >  2:1186607/1‑90 (MQ=255)                                                                     gTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCgg            <  1:957373/90‑1 (MQ=255)                                                                           gTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGa      <  2:915102/90‑1 (MQ=255)                                                                             cAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGt    <  1:5230/90‑1 (MQ=255)                                                                               gCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTgg  >  1:286744/1‑90 (MQ=255)                                                                               gCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTgg  >  1:1106321/1‑90 (MQ=255)                                                                                   |                                                                                      CTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGG  >  NZ_CP009273/4153734‑4153901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑