Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,527,151	T→C	Q87Q (CAA→CAG)	nanS ←	9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,527,151	0	T	C	100.0%	212.2 / NA	60	Q87Q (CAA→CAG)	nanS	9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/25);  total (35/25)

GAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTTTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTAA  >  NZ_CP009273/4527072‑4527236                                                                                |                                                                                      gAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCg                                                                             >  1:644669/1‑90 (MQ=255)  aaTCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGt                                                                            >  1:1159172/1‑90 (MQ=255)       cTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCa                                                                       >  2:981383/1‑90 (MQ=255)       cTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCa                                                                       >  2:639811/1‑90 (MQ=255)       cTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCa                                                                       >  1:1165757/1‑90 (MQ=255)        tGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCag                                                                      <  1:531205/90‑1 (MQ=255)            ttATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa                                                                  <  2:725763/90‑1 (MQ=255)              aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                >  1:407101/1‑90 (MQ=255)                 aGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCag                                                                      <  2:645920/81‑1 (MQ=255)                 aGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCag                                                                      >  1:645920/1‑81 (MQ=255)                   gAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGAt                                                           >  2:530962/1‑90 (MQ=255)                   gAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGAt                                                           <  2:505515/90‑1 (MQ=255)                       gAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAAcc                                                       <  2:1165757/90‑1 (MQ=255)                            gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  >  2:343519/1‑90 (MQ=255)                            gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  1:669137/90‑1 (MQ=255)                              aGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCata                                                <  2:873761/90‑1 (MQ=255)                              aGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCata                                                <  2:583358/90‑1 (MQ=255)                              aGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCata                                                <  1:90562/90‑1 (MQ=255)                                      ccGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAAc                                        <  2:1006449/90‑1 (MQ=255)                                       cGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACa                                       <  1:89019/90‑1 (MQ=255)                                       cGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACa                                       <  2:64848/90‑1 (MQ=255)                                         tGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATc                                     >  1:365968/1‑90 (MQ=255)                                         tGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATc                                     <  2:429115/90‑1 (MQ=255)                                         tGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATc                                     >  2:1056264/1‑90 (MQ=255)                                             atatGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCgtgt                                 >  1:722009/1‑90 (MQ=255)                                             atatGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCgtgt                                 <  2:1173746/90‑1 (MQ=255)                                                 gCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGc                             <  1:21570/90‑1 (MQ=255)                                                 gCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGc                             <  1:879956/90‑1 (MQ=255)                                                 gCAGTGCCTGGCCAACGGTGACGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGc                             <  1:201042/90‑1 (MQ=255)                                                    gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa                                                                  >  2:193756/1‑50 (MQ=255)                                                    gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa                                                                  <  1:193756/50‑1 (MQ=255)                                                    gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGt                          >  1:1213896/1‑90 (MQ=255)                                                     tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCag                                                                      <  1:255482/45‑1 (MQ=255)                                                     tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCag                                                                      >  2:255482/1‑45 (MQ=255)                                                     tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACa                                       <  1:882024/76‑1 (MQ=255)                                                     tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACa                                       >  2:882024/1‑76 (MQ=255)                                                     tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTg                         >  2:648757/1‑90 (MQ=255)                                                     tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTg                         >  2:124576/1‑90 (MQ=255)                                                     tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTg                         >  2:517500/1‑90 (MQ=255)                                                      gCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGa                        >  2:588013/1‑90 (MQ=255)                                                         tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa                                                                  >  2:83024/1‑45 (MQ=255)                                                         tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa                                                                  <  1:83024/45‑1 (MQ=255)                                                         tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc                     >  1:905571/1‑90 (MQ=255)                                                         tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc                     >  1:65178/1‑90 (MQ=255)                                                            ccAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGt                  <  1:639811/90‑1 (MQ=255)                                                            ccAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGt                  <  1:241623/90‑1 (MQ=255)                                                                cGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGaa              >  2:327031/1‑90 (MQ=255)                                                                    gCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGaataat          >  1:538251/1‑90 (MQ=255)                                                                    gCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGaataat          >  1:1100414/1‑90 (MQ=255)                                                                     ccGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATg         >  1:931481/1‑90 (MQ=255)                                                                            gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACa                                       <  2:480470/53‑1 (MQ=255)                                                                            gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACa                                       >  1:480470/1‑53 (MQ=255)                                                                            gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTgg                               <  2:42943/61‑1 (MQ=255)                                                                            gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTgg                               >  1:42943/1‑61 (MQ=255)                                                                            gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  2:598791/1‑90 (MQ=255)                                                                            gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  1:434447/1‑90 (MQ=255)                                                                            gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  2:1099653/1‑90 (MQ=255)                                                                            gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  2:1087424/1‑90 (MQ=255)                                                                            gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  1:1090258/1‑90 (MQ=255)                                                                            gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  2:847414/1‑90 (MQ=255)                                                                                |                                                                                      GAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTTTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTAA  >  NZ_CP009273/4527072‑4527236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑