Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	568,752	A→G	G71G (GGA→GGG)	ybcO →	DUF1364 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	568,752	0	A	G	100.0%	144.2 / NA	42	G71G (GGA→GGG)	ybcO	DUF1364 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/26);  total (16/26)

TACGAAACCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGATATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATACCTAC  >  NZ_CP009273/568665‑568838                                                                                        |                                                                                       tACGAACCCGCCAGCCCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGgta                                                                                      <  1:760313/90‑1 (MQ=255) tACGAACCCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGgta                                                                                      <  1:95652/90‑1 (MQ=255)    gAAACCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGc                                                                                   >  1:496074/1‑90 (MQ=255)    gAAACCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGc                                                                                   >  2:1169382/1‑90 (MQ=255)        ccGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCaaaa                                                                               >  1:1171120/1‑90 (MQ=255)           ccAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGaa                                                                            <  1:1171933/90‑1 (MQ=255)                ccTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATgcgc                                                                       <  2:1171120/90‑1 (MQ=255)                  tGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATgcgcgc                                                                     <  1:673677/90‑1 (MQ=255)                   gATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCt                                                                    >  1:470700/1‑90 (MQ=255)                   gATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCt                                                                    >  2:556209/1‑90 (MQ=255)                    aTTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTg                                                                   <  2:534009/90‑1 (MQ=255)                        ccaccaTTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAg                                                               <  2:841451/90‑1 (MQ=255)                             aTTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGTTATg                                                          >  1:890035/1‑90 (MQ=255)                             aTTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATg                                                          <  2:18406/90‑1 (MQ=255)                                gCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCg                                                       <  1:661704/90‑1 (MQ=255)                                   tGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCgaga                                                    <  2:1067289/90‑1 (MQ=255)                                   tGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCgaga                                                    <  1:1167754/90‑1 (MQ=255)                                     ttCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGa                                                                 >  1:584802/1‑75 (MQ=255)                                     ttCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGa                                                                 <  2:584802/75‑1 (MQ=255)                                     ttCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAac                                                  >  2:40752/1‑90 (MQ=255)                                     ttCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAac                                                  >  2:1083214/1‑90 (MQ=255)                                       ctgcctgcCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAacac                                                <  2:985070/90‑1 (MQ=255)                                         gcctgccACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAg                                              >  1:1018336/1‑90 (MQ=255)                                         gcctgccACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAg                                              >  2:113925/1‑90 (MQ=255)                                             gccACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTa                                          >  1:387696/1‑90 (MQ=255)                                               cACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATc                                        <  2:1134282/90‑1 (MQ=255)                                                   acgaAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGc                                    <  1:555348/90‑1 (MQ=255)                                                   acgaAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGc                                    >  2:902297/1‑90 (MQ=255)                                                       aaTCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATc                                        <  2:1212573/82‑1 (MQ=255)                                                       aaTCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATc                                        >  1:1212573/1‑82 (MQ=255)                                                         tCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAag                              <  1:45113/90‑1 (MQ=255)                                                         tCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAag                              <  1:652409/90‑1 (MQ=255)                                                           gACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAagag                            >  2:791620/1‑90 (MQ=255)                                                           gACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAaga                             >  2:568974/1‑89 (MQ=255)                                                           gACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAaga                             <  1:568974/89‑1 (MQ=255)                                                            aCCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAgg                           <  2:1130165/90‑1 (MQ=255)                                                             ccgccgCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAggg                          <  1:388744/90‑1 (MQ=255)                                                                ccgcACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGGTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGt                       <  1:372077/90‑1 (MQ=255)                                                                     cgcaTTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGttatta                  <  1:994808/90‑1 (MQ=255)                                                                       caTTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAg                <  2:831039/90‑1 (MQ=255)                                                                           ttgttgACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGt            <  2:1018336/90‑1 (MQ=255)                                                                              ttgACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGaa         <  2:440886/90‑1 (MQ=255)                                                                              ttgACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGaa         <  2:387696/90‑1 (MQ=255)                                                                                     tGGGTATGCAAAAGAATGCGCGCTGGAAGTTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATACCTAc  <  2:890035/90‑1 (MQ=255)                                                                                        |                                                                                       TACGAAACCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGATATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATACCTAC  >  NZ_CP009273/568665‑568838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑