Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	661,892	A→C	G594G (GGT→GGG)	mrdA ←	peptidoglycan DD‑transpeptidase MrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	661,892	0	A	C	100.0%	191.7 / NA	55	G594G (GGT→GGG)	mrdA	peptidoglycan DD‑transpeptidase MrdA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/25);  total (30/25)

TTCCGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCACCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGC  >  NZ_CP009273/661805‑661976                                                                                        |                                                                                     ttCCGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCaccccc                                                                                    >  2:1054256/1‑90 (MQ=255)   ccgccgGCAGATCGGTGTTGTTATCCCCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGt                                                                                  <  1:682221/90‑1 (MQ=255)   ccGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCGTCAGTGTACCAACCGCCGGACCCGCACCCCCGt                                                                                  <  2:368584/90‑1 (MQ=255)      caggcagATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTtct                                                                               >  1:537225/1‑90 (MQ=255)        ggcagATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTcc                                                                             <  1:874480/90‑1 (MQ=255)          cagATCGGTGTTGTTATCCCCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAg                                                                           <  1:761940/90‑1 (MQ=255)            gATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGaa                                                                         >  1:864906/1‑90 (MQ=255)                 gtgtTGTTATCCCCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATg                                                                    <  1:54221/90‑1 (MQ=255)                      gttATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGt                                                                                  >  1:1173930/1‑71 (MQ=255)                      gttATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGt                                                                                  <  2:1173930/71‑1 (MQ=255)                      gttATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGAc                                                               >  1:407651/1‑90 (MQ=255)                         aTCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGc                                                            <  2:743656/90‑1 (MQ=255)                           cACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCa                                                          >  1:824298/1‑90 (MQ=255)                               cAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTg                                                      >  2:1232416/1‑90 (MQ=255)                                aGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                     >  1:388548/1‑90 (MQ=255)                                aGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                     >  1:886266/1‑90 (MQ=255)                                aGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                     >  1:1168602/1‑90 (MQ=255)                                  cATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg                                                   <  1:43032/90‑1 (MQ=255)                                  cATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg                                                   <  1:126025/90‑1 (MQ=255)                                  cATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg                                                   <  2:480619/90‑1 (MQ=255)                                      aTGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAttg                                               >  1:321171/1‑90 (MQ=255)                                      aTGTGGTCGAGGATCTGGCACATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAttg                                               >  1:1139263/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACATCCACTTGCGTATTGTTGTa                                          >  1:146396/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                     <  2:1010199/79‑1 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                     >  1:1010199/1‑79 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg                                                   <  1:964211/81‑1 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg                                                   <  2:653339/81‑1 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg                                                   >  2:964211/1‑81 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg                                                   >  1:653339/1‑81 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                          >  2:86979/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                          >  1:1031725/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                          >  2:100705/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                          >  2:734241/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                          >  1:394542/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                          >  1:196759/1‑90 (MQ=255)                                            tCGAGGATCTGGCGCATCAGTGTCCCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTAt                                         <  1:929776/90‑1 (MQ=255)                                            tCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTAt                                         <  1:1011803/90‑1 (MQ=255)                                            tCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTAt                                         <  2:1131933/90‑1 (MQ=255)                                                  aTCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCa                                   >  1:44701/1‑90 (MQ=255)                                                     tGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGt                                                                                  <  1:449281/40‑1 (MQ=255)                                                     tGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGt                                                                                  >  2:449281/1‑40 (MQ=255)                                                         gcATCAGTGTACCAACCGCCGGACCCGCCCCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCgg                            <  1:1155864/90‑1 (MQ=255)                                                              aGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGtcatc                       <  2:1182132/90‑1 (MQ=255)                                                              aGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGtcatc                       <  2:715340/90‑1 (MQ=255)                                                                      aaCCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTtgtg               >  1:1140349/1‑90 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGcc                                                           >  2:1141874/1‑43 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGcc                                                           <  1:1141874/43‑1 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc            <  2:192362/90‑1 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc            <  1:446312/90‑1 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc            >  2:1033681/1‑90 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc            >  1:494855/1‑90 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc            >  1:549017/1‑90 (MQ=255)                                                                                 ccGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAAc    >  2:683418/1‑90 (MQ=255)                                                                                   gCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt                                                 >  1:962870/1‑43 (MQ=255)                                                                                   gCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt                                                 <  2:962870/43‑1 (MQ=255)                                                                                   gCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGc  <  2:1168602/90‑1 (MQ=255)                                                                                        |                                                                                     TTCCGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCACCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGC  >  NZ_CP009273/661805‑661976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑