Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	674,442	T→G	T141P (ACT→CCT)	ybeT ←	tetratricopeptide repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	674,442	0	T	G	100.0%	174.9 / NA	50	T141P (ACT→CCT)	ybeT	tetratricopeptide repeat protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/28);  total (22/28)

GTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAG  >  NZ_CP009273/674355‑674523                                                                                         |                                                                                  gttgttTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGgat                                                                                 >  1:1193561/1‑90 (MQ=255) gttgttTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGgat                                                                                 >  1:842275/1‑90 (MQ=255)   tgttTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGatat                                                                               <  2:369964/90‑1 (MQ=255)   tgttTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGatat                                                                               <  2:1043415/90‑1 (MQ=255)   tgttTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGatat                                                                               >  1:926508/1‑90 (MQ=255)   tgttTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGatat                                                                               <  1:655316/90‑1 (MQ=255)    gttTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATAtt                                                                              <  1:369677/90‑1 (MQ=255)      ttGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtga                                                                            >  2:1227738/1‑90 (MQ=255)         gCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagca                                                                         <  2:558710/90‑1 (MQ=255)         gCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagca                                                                         <  2:511106/90‑1 (MQ=255)         gCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagca                                                                         <  2:275106/90‑1 (MQ=255)          cGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagcat                                                                        <  2:1202851/90‑1 (MQ=255)          cGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagcat                                                                        <  2:1159743/90‑1 (MQ=255)          cGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagcat                                                                        <  2:722419/90‑1 (MQ=255)           ggTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagcatg                                                                       <  2:181709/90‑1 (MQ=255)           ggTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagcatg                                                                       <  2:296029/90‑1 (MQ=255)           ggTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagcatg                                                                       >  1:1199073/1‑90 (MQ=255)              cAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagc                                                                          >  2:1222426/1‑84 (MQ=255)              cAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagc                                                                          <  1:1222426/84‑1 (MQ=255)                     aaCCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCt                                                             <  1:1011825/90‑1 (MQ=255)                          tttttGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGGTCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTc                                                        >  2:1132814/1‑90 (MQ=255)                          tttttGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTc                                                        >  2:338255/1‑90 (MQ=255)                                agaTTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAgctgc                                                  <  2:34150/90‑1 (MQ=255)                                aagatCAAAAAGGTTAAAAATTAATCCCTGGGCCTTTTTTTAAGAT‑AAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAgctgc                                                  <  1:241432/86‑1 (MQ=255)                                 gaTTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAgctgct                                                 <  1:911830/90‑1 (MQ=255)                                            gTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCa                                      >  1:249982/1‑90 (MQ=255)                                              tAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCaga                                    >  1:30267/1‑90 (MQ=255)                                              tAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCaga                                    >  2:839478/1‑90 (MQ=255)                                                 c‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGaa                                 <  2:586397/90‑1 (MQ=255)                                                 c‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGaa                                 >  1:315314/1‑90 (MQ=255)                                                   aTTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAAt                                <  1:728991/90‑1 (MQ=255)                                                   aTTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAAt                                <  1:338255/90‑1 (MQ=255)                                                    ttAATACCTGAGCCATTTTTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAAtt                               <  2:738831/90‑1 (MQ=255)                                                      aaTACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGc                             >  1:239458/1‑90 (MQ=255)                                                      aaTACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGc                             >  1:1034018/1‑90 (MQ=255)                                                      aaTACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATCGc                             <  2:147585/90‑1 (MQ=255)                                                           cTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTAc                        <  2:842275/90‑1 (MQ=255)                                                            tGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagcat                                                                        <  1:1027343/41‑1 (MQ=255)                                                            tGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagcat                                                                        >  2:1027343/1‑41 (MQ=255)                                                            tGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACg                       >  2:10576/1‑90 (MQ=255)                                                            tGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACg                       >  1:272572/1‑90 (MQ=255)                                                            tGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACg                       >  2:1001276/1‑90 (MQ=255)                                                            tGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACg                       >  1:491259/1‑90 (MQ=255)                                                             gAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACgg                      >  1:331192/1‑90 (MQ=255)                                                              aGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACggg                     >  1:1036011/1‑90 (MQ=255)                                                                  aTTTCTGTAGATCAAACCTAAAGGATATTgagcatgagcat                                                                  >  2:944624/1‑41 (MQ=255)                                                                  aTTTCTGTAGATCAAACCTAAAGGATATTgagcatgagcat                                                                  <  1:944624/41‑1 (MQ=255)                                                                          aGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGa         <  1:839478/90‑1 (MQ=255)                                                                          aGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGa         <  1:1001276/90‑1 (MQ=255)                                                                                aaCCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCa   >  1:794966/1‑90 (MQ=255)                                                                                aaCCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCa   >  1:499363/1‑90 (MQ=255)                                                                                 aCCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAg  <  2:331192/90‑1 (MQ=255)                                                                                         |                                                                                  GTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAAC‑ATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAG  >  NZ_CP009273/674355‑674523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑