Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	886,189	A→G	G7G (GGT→GGC)	grxA ←	glutaredoxin 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	886,189	0	A	G	100.0%	144.6 / NA	42	G7G (GGT→GGC)	grxA	glutaredoxin 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/15);  total (27/15)

ACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGAAAGT  >  NZ_CP009273/886103‑886270                                                                                       |                                                                                  aCATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCa                                                                                <  1:981647/90‑1 (MQ=255) aCATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCa                                                                                <  1:263310/90‑1 (MQ=255)    tactgatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCaaaa                                                                             >  2:769358/1‑90 (MQ=255)      ctgatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAAt                                                                           >  2:809805/1‑90 (MQ=255)        gatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATaa                                                                         >  1:805811/1‑90 (MQ=255)        gatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATaa                                                                         >  2:124564/1‑90 (MQ=255)        gatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATaa                                                                         >  2:483216/1‑90 (MQ=255)          tactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACg                                                                       >  1:616424/1‑90 (MQ=255)           actgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACgg                                                                      >  2:1083772/1‑90 (MQ=255)              gaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGttt                                                                   >  1:134772/1‑90 (MQ=255)              gaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGttt                                                                   <  2:781859/90‑1 (MQ=255)                aaaTCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGc                                                                 <  1:157304/90‑1 (MQ=255)                  atcatcGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCat                                                               <  2:790868/90‑1 (MQ=255)                    catcGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCatta                                                             >  1:562227/1‑90 (MQ=255)                      tcGCGTTCATTGCTCGATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCattatt                                                           >  1:956437/1‑90 (MQ=255)                          gTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTtctc                                                       >  2:877449/1‑90 (MQ=255)                                 gCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCAt                                                <  2:968679/90‑1 (MQ=255)                                  cTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATa                                               <  1:690292/90‑1 (MQ=255)                                  cTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATa                                               <  1:1165508/90‑1 (MQ=255)                                      aTTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt                                           >  2:313114/1‑90 (MQ=255)                                       tttCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCAAAGAttt                                          <  1:1199620/90‑1 (MQ=255)                                           tcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGc                                      >  2:757718/1‑90 (MQ=255)                                           tcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGc                                      >  1:379137/1‑90 (MQ=255)                                            cAGCCAGATCTTCTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGcc                                     <  2:462723/90‑1 (MQ=255)                                                          gcacgcacACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGc                       <  2:805811/90‑1 (MQ=255)                                                            acgcacACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTa                     >  2:1044537/1‑90 (MQ=255)                                                              gcacACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTaaa                   >  1:1032869/1‑90 (MQ=255)                                                                       aaGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCg          >  2:305656/1‑90 (MQ=255)                                                                         gggCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTAt                                        <  1:1185130/58‑1 (MQ=255)                                                                         gggCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTAt                                        >  2:1185130/1‑58 (MQ=255)                                                                           gCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGcc                                     <  2:583881/59‑1 (MQ=255)                                                                           gCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGcc                                     >  1:583881/1‑59 (MQ=255)                                                                           gCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGa      >  1:693998/1‑90 (MQ=255)                                                                           gCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGa      >  1:1051348/1‑90 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCAt                                                <  2:420700/46‑1 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCAt                                                >  1:420700/1‑46 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGaaa    >  2:674236/1‑90 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGaaa    >  2:315849/1‑90 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGaaa    >  1:525906/1‑90 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGaaa    >  1:69726/1‑90 (MQ=255)                                                                               cccGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGAAAGt  >  1:319112/1‑90 (MQ=255)                                                                               cccGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGAAAGt  <  1:562082/90‑1 (MQ=255)                                                                                       |                                                                                  ACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGAAAGT  >  NZ_CP009273/886103‑886270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑