Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,062,210	T→C	G390G (GGT→GGC)	agp →	bifunctional glucose‑1‑phosphatase/inositol phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,062,210	0	T	C	100.0%	107.4 / NA	32	G390G (GGT→GGC)	agp	bifunctional glucose‑1‑phosphatase/inositol phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/11);  total (21/11)

ATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAA  >  NZ_CP009273/1062128‑1062287                                                                                   |                                                                              aTCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGa                                                                        >  2:560619/1‑90 (MQ=255)    agagTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAg                                                                     <  2:817743/90‑1 (MQ=255)     gagTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGGGTGACGCTGGAATTAAGCGGCTGCCCGATAGa                                                                    >  1:39339/1‑90 (MQ=255)       gTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACg                                                                  >  2:130801/1‑90 (MQ=255)       gTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACg                                                                  >  1:929435/1‑90 (MQ=255)       gTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACg                                                                  >  1:429677/1‑90 (MQ=255)             aaCAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATg                                                            <  1:914193/90‑1 (MQ=255)                 gTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGttt                                                        >  2:1101542/1‑90 (MQ=255)                   tACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCt                                                      >  1:402874/1‑90 (MQ=255)                         aTGCCGATGCGTTAGCCCTGCTGGCTCCGGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGTCGCTGATGGTTTCTGCCCGa                                                <  1:1043688/90‑1 (MQ=255)                         aTGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGa                                                >  1:276465/1‑90 (MQ=255)                         aTGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGa                                                >  1:718818/1‑90 (MQ=255)                           gCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATg                                              >  1:822971/1‑90 (MQ=255)                           gCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATg                                              >  1:898779/1‑90 (MQ=255)                              gATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGAt                                           >  1:580641/1‑90 (MQ=255)                               aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                          <  2:822971/90‑1 (MQ=255)                               aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                          <  1:455861/90‑1 (MQ=255)                               aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                          <  2:402874/90‑1 (MQ=255)                               aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                          <  2:276465/90‑1 (MQ=255)                               aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                          <  2:1185605/90‑1 (MQ=255)                                           tGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCg                              >  1:49727/1‑90 (MQ=255)                                                    cTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTtgaatg                     >  1:1124037/1‑90 (MQ=255)                                                      gcgcAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTtgaatgaa                   >  2:835071/1‑90 (MQ=255)                                                           gCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGt              >  1:1135489/1‑90 (MQ=255)                                                           gCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGt              >  1:1123459/1‑90 (MQ=255)                                                             gtgtGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGa            >  1:1102804/1‑90 (MQ=255)                                                               gtgACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                          <  2:1000974/58‑1 (MQ=255)                                                               gtgACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                          >  1:1000974/1‑58 (MQ=255)                                                                 gACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATa        >  2:1125773/1‑90 (MQ=255)                                                                     cTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAg    <  2:39339/90‑1 (MQ=255)                                                                     cTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAg    <  1:279566/90‑1 (MQ=255)                                                                       ggAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGaa  >  1:12743/1‑90 (MQ=255)                                                                                   |                                                                              ATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAA  >  NZ_CP009273/1062128‑1062287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑