Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	98,192	T→C	I314T (ATT→ACT)	murC →	UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	98,192	0	T	C	100.0%	94.4 / NA	28	I314T (ATT→ACT)	murC	UDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/9);  total (19/9)

TGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTAAAA  >  NZ_CP009273/98112‑98278                                                                                 |                                                                                       tGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGgc                                                                               <  2:73627/90‑1 (MQ=255)   cgcCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGgcgc                                                                             >  1:149105/1‑90 (MQ=255)   cgcCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGgcgc                                                                             >  2:1237709/1‑90 (MQ=255)   cgcCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCCACTTTGCGGgcgc                                                                             >  2:696191/1‑90 (MQ=255)                  aCGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAgg                                                              >  2:658913/1‑90 (MQ=255)                      gcTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGAc                                                          >  2:514689/1‑90 (MQ=255)                      gcTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGAc                                                          >  1:607802/1‑90 (MQ=255)                       cTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACt                                                         >  2:322579/1‑90 (MQ=255)                             gccgcAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGc                                                   <  2:94736/90‑1 (MQ=255)                              ccgcAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGcc                                                  >  2:1197907/1‑90 (MQ=255)                              ccgcAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGcc                                                  >  1:411275/1‑90 (MQ=255)                                  aGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGtct                                              <  2:183415/90‑3 (MQ=255)                                        ggttgcggttgcTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGAttt                                        >  2:480000/1‑90 (MQ=255)                                              ggttgcTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGGTTTGATTTCCTCgg                                  >  1:323153/1‑90 (MQ=255)                                                    tACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAAtt                            >  1:492024/1‑90 (MQ=255)                                                      cGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTcc                          <  2:446694/90‑1 (MQ=255)                                                             gggCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGa                   >  1:1160100/1‑90 (MQ=255)                                                             gggCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGa                   >  1:428435/1‑90 (MQ=255)                                                               gCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGc                 >  2:742125/1‑90 (MQ=255)                                                               gCATTGACGACGAGGCCACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGc                 <  1:696191/90‑1 (MQ=255)                                                                cATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGcc                >  2:654508/1‑90 (MQ=255)                                                                cATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGcc                >  1:768003/1‑90 (MQ=255)                                                                  ttGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAg              >  2:612823/1‑90 (MQ=255)                                                                  ttGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAg              <  2:768003/90‑1 (MQ=255)                                                                     acgacgAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGa           >  1:360508/1‑90 (MQ=255)                                                                      cgacgaGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGaa          <  2:133459/90‑1 (MQ=255)                                                                      cgacgaGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGaa          <  1:742125/90‑1 (MQ=255)                                                                              cTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTaaaa  <  2:322119/90‑1 (MQ=255)                                                                                 |                                                                                       TGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTAAAA  >  NZ_CP009273/98112‑98278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑