Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,259,173	A→G	T2A (ACC→GCC)	hemA →	glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,259,173	0	A	G	97.5%	132.3 / NA	40	T2A (ACC→GCC)	hemA	glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (26/13);  total (26/14)
Fisher's exact test for biased strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCA  >  NZ_CP009273/1259086‑1259258                                                                                        |                                                                                      gggCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGCCTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGcc                                                                                     <  1:779630/90‑1 (MQ=255) gggCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGcc                                                                                     >  2:986964/1‑90 (MQ=255) gggCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGcc                                                                                     >  2:212047/1‑90 (MQ=255) gggCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGcc                                                                                     <  2:1157468/90‑1 (MQ=255)                  gATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTAt                                                                    >  1:197058/1‑90 (MQ=255)                  gATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTAt                                                                    >  2:825138/1‑90 (MQ=255)                  gATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTAt                                                                    >  2:660375/1‑90 (MQ=255)                  gATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTAt                                                                    >  1:400462/1‑90 (MQ=255)                    tCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCACCGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCa                                                                  <  1:863834/90‑1 (MQ=255)                    tCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACt                                                                          <  2:854658/82‑1 (MQ=255)                    tCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACt                                                                          >  1:854658/1‑82 (MQ=255)                    tCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCa                                                                  >  1:44763/1‑90 (MQ=255)                    tCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCa                                                                  <  2:1058743/90‑1 (MQ=255)                     cTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCaa                                                                 <  1:660375/90‑1 (MQ=255)                            acccTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCGCTTTTAGCACTCGGTATCAACCATaaa                                                          >  2:754971/3‑90 (MQ=255)                            aTCCTGTATGATGCAGGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATaaa                                                          >  1:335512/1‑90 (MQ=255)                            aTCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATaaa                                                          >  1:226134/1‑90 (MQ=255)                            aTCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATaaa                                                          >  2:732094/1‑90 (MQ=255)                            aTCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATaaa                                                          >  1:929002/1‑90 (MQ=255)                              ccTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAAc                                                        >  2:154653/1‑90 (MQ=255)                                 gTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCa                                                                  >  2:805113/1‑77 (MQ=255)                                 gTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCa                                                                  <  1:805113/77‑1 (MQ=255)                                 gTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGc                                                     >  1:790807/1‑90 (MQ=255)                                      atgCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCa                                                    <  2:696328/86‑1 (MQ=255)                                      atgCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCa                                                    >  1:696328/1‑86 (MQ=255)                                       tgCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGt                                               >  2:461479/1‑90 (MQ=255)                                           aGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGc                                                     >  2:698749/1‑80 (MQ=255)                                           aGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGc                                                     <  1:698749/80‑1 (MQ=255)                                           aGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCg                                           >  1:923779/1‑90 (MQ=255)                                                         aTCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACgtgt                             >  1:517234/1‑90 (MQ=255)                                                         aTCAACGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACgtgt                             >  1:728015/1‑90 (MQ=255)                                                             aCGTTGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCg                         >  1:968652/1‑90 (MQ=255)                                                                 tGGTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGtttt                     >  1:1208479/1‑90 (MQ=255)                                                                   gTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCgaga                                   >  1:291415/1‑74 (MQ=255)                                                                   gTATTATTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCgaga                                   <  2:291415/74‑1 (MQ=255)                                                                    tattatTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGc                  <  2:268038/90‑1 (MQ=255)                                                                    tattatTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGc                  <  1:577676/90‑1 (MQ=255)                                                                    tattatTTCCCGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGc                  <  1:451628/90‑1 (MQ=255)                                                                             ccGCAGACATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGc         >  2:894024/1‑90 (MQ=255)                                                                                    cATGGCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCa  <  2:790807/90‑1 (MQ=255)                                                                                        |                                                                                      GGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCA  >  NZ_CP009273/1259086‑1259258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑