Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,394,663	T→C	G456G (GGA→GGG)	abgT ←	p‑aminobenzoyl‑glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,394,663	0	T	C	100.0%	83.4 / NA	25	G456G (GGA→GGG)	abgT	p‑aminobenzoyl‑glutamate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/10);  total (15/10)

CATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCGCAA  >  NZ_CP009273/1394577‑1394745                                                                                       |                                                                                   cATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCa                                                                                 >  2:532563/1‑90 (MQ=255)  aTACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCaa                                                                                <  1:657697/90‑1 (MQ=255)           aaaaGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTg                                                                       <  1:1003072/90‑1 (MQ=255)                 tAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACaa                                                                 <  2:473919/90‑1 (MQ=255)                           gggCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAgg                                                             >  2:265058/1‑84 (MQ=255)                           gggCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAgg                                                             <  1:265058/84‑1 (MQ=255)                             gCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCg                                                     >  2:807758/1‑90 (MQ=255)                              cAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCgg                                                    <  1:43811/90‑1 (MQ=255)                              cAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCgg                                                    >  1:458803/1‑90 (MQ=255)                              cAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCgg                                                    >  2:507056/1‑90 (MQ=255)                              cAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCgg                                                    <  1:1082606/90‑1 (MQ=255)                                     aaCGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTaaa                                             >  1:320508/1‑90 (MQ=255)                                     aaCGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTaaa                                             >  1:236246/1‑90 (MQ=255)                                             gtaagtaCCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATAc                                     >  2:323907/1‑90 (MQ=255)                                                       aGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCg                           >  2:194652/1‑90 (MQ=255)                                                         tttCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGc                         <  1:710519/90‑1 (MQ=255)                                                            cgcgTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAAt                      <  1:807758/90‑1 (MQ=255)                                                                  tGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGaaa                >  1:953264/1‑90 (MQ=255)                                                                         tAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGAtt         <  2:458803/90‑1 (MQ=255)                                                                            cGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTgc      >  2:246242/1‑90 (MQ=255)                                                                             gTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGc                                          >  2:164011/1‑53 (MQ=255)                                                                             gTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGc                                          <  1:164011/53‑1 (MQ=255)                                                                             gTTGCAGGAACCCAAGAAAAAGTGGAACAAAAGGAGATACCGGCGCTAAAGGAAATACGGATGAGGCGGCAATACGAAAGAGGATTTgcg     >  1:1122017/1‑90 (MQ=255)                                                                              ttGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTgcgc    >  2:814373/1‑90 (MQ=255)                                                                                gCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGATTCGGCAATACGAAAGAGGATTTGCGCaa  >  1:987229/1‑90 (MQ=255)                                                                                       |                                                                                   CATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCGCAA  >  NZ_CP009273/1394577‑1394745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑