Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,494,123	T→C	E195G (GAA→GGA)	ydcK ←	YdcK family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,494,123	0	T	C	100.0%	114.8 / NA	34	E195G (GAA→GGA)	ydcK	YdcK family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/13);  total (21/13)

ATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGG  >  NZ_CP009273/1494048‑1494206                                                                            |                                                                                    aTGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCaaaa                                                                       >  1:1217547/1‑90 (MQ=255) aTGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCaaaa                                                                       >  1:904614/1‑90 (MQ=255)     cAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCaa                                                                   >  2:130324/1‑90 (MQ=255)       gCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCaaaa                                                                 >  2:454535/1‑90 (MQ=255)                 gcgcGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGgcgc                                                       <  1:130324/90‑1 (MQ=255)                 gcgcGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGgcgc                                                       >  1:357753/1‑90 (MQ=255)                 gcgcGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGgcgc                                                       >  2:192865/1‑90 (MQ=255)                   gcgTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGa                                                     <  1:822888/90‑1 (MQ=255)                            tAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATa                                            <  2:916004/90‑1 (MQ=255)                            tAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATa                                            <  2:528198/90‑1 (MQ=255)                                  ttAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCa                                      >  2:1165568/1‑90 (MQ=255)                                  ttAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCa                                      >  1:1184030/1‑90 (MQ=255)                                     gcgcAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATAc                                   >  1:415511/1‑90 (MQ=255)                                          aTCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGgac                              >  2:268165/1‑90 (MQ=255)                                                   ccAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATc                     <  2:88427/90‑1 (MQ=255)                                                   ccAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATc                     <  2:1047633/90‑1 (MQ=255)                                                     aGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGc                   <  1:821477/90‑1 (MQ=255)                                                      gACATTGTTCTCTTCATTACCTCCAATACTGGCAGAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGcc                  >  2:196233/1‑90 (MQ=255)                                                      gACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGcc                  >  2:494169/1‑90 (MQ=255)                                                          ttGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATAc                                   <  1:461879/69‑1 (MQ=255)                                                          ttGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATAc                                   >  2:461879/1‑69 (MQ=255)                                                          ttGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATaa              >  2:281767/1‑90 (MQ=255)                                                          ttGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATaa              >  2:675577/1‑90 (MQ=255)                                                           tGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATaaa             >  2:777929/1‑90 (MQ=255)                                                            gTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAAt            >  2:236085/1‑90 (MQ=255)                                                            gTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAAt            >  1:543873/1‑90 (MQ=255)                                                               ctctTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTtg         <  1:454535/90‑1 (MQ=255)                                                                 ctTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTtgtg       >  1:1152785/1‑90 (MQ=255)                                                                 ctTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTtgtg       <  2:770909/90‑1 (MQ=255)                                                                   tCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGcc     <  2:765908/90‑1 (MQ=255)                                                                    cATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCt    <  2:556572/90‑1 (MQ=255)                                                                      ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATaa              <  2:1149995/78‑1 (MQ=255)                                                                      ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATaa              >  1:1149995/1‑78 (MQ=255)                                                                      ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtgg  >  1:714032/1‑90 (MQ=255)                                                                            |                                                                                    ATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGG  >  NZ_CP009273/1494048‑1494206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑