Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,502,076	A→G	N347D (AAC→GAC)	rlhA →	23S rRNA 5‑hydroxycytidine C2501 synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,502,076	0	A	G	100.0%	72.2 / NA	22	N347D (AAC→GAC)	rlhA	23S rRNA 5‑hydroxycytidine C2501 synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/17);  total (5/17)

TGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGG  >  NZ_CP009273/1501993‑1502156                                                                                    |                                                                                 tGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACgtgt                                                                            <  1:40529/90‑1 (MQ=255) tGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACgtgt                                                                            <  1:97525/90‑1 (MQ=255)  gCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGtt                                                                           >  1:697395/1‑90 (MQ=255)      gTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGAtt                                                                       <  2:544480/90‑1 (MQ=255)       ttggCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTa                                                                      <  2:750130/88‑1 (MQ=255)              agtagtGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGa                                                               <  2:933218/90‑1 (MQ=255)                          ggCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGttt                                                   >  1:854480/1‑90 (MQ=255)                          ggCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGttt                                                   >  2:1164590/1‑90 (MQ=255)                               aaGATCATCTCGATGTTGCCGTTCCCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTg                                              <  1:407942/90‑1 (MQ=255)                               aaGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTg                                              <  2:697395/90‑1 (MQ=255)                               aaGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTg                                              <  2:661528/90‑1 (MQ=255)                                      tctcGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATAc                                       <  1:1164590/90‑1 (MQ=255)                                                   tttcccGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAAcc                          <  1:923797/86‑1 (MQ=255)                                                        cGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGgaga                     <  2:338381/90‑1 (MQ=255)                                                          aGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGaaa                   <  2:208087/90‑1 (MQ=255)                                                             cACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATc                <  2:854480/90‑1 (MQ=255)                                                               cTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAg              <  2:379257/90‑1 (MQ=255)                                                               cTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAg              <  2:974648/90‑1 (MQ=255)                                                                   cAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTAcc          <  1:53174/90‑1 (MQ=255)                                                                   cAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTAcc          <  1:213034/90‑1 (MQ=255)                                                                           gATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACTGCGTCTgg  >  2:877675/1‑90 (MQ=255)                                                                           gATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTgg  >  1:1116716/1‑90 (MQ=255)                                                                                    |                                                                                 TGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGG  >  NZ_CP009273/1501993‑1502156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑