| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,505,430 | A→G | K391E (AAA→GAA) | ydcR → | PLP‑dependent aminotransferase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,505,430 | 0 | A | G | 93.3% | 38.6 / ‑5.0 | 15 | K391E (AAA→GAA) | ydcR | PLP‑dependent aminotransferase family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (8/6); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTG > NZ_CP009273/1505343‑1505516 | gCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGgaa > 1:1063619/1‑90 (MQ=255) ttCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtcatc < 2:432679/90‑1 (MQ=255) gCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATg > 2:1192668/1‑90 (MQ=255) ccTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGa < 2:13454/90‑1 (MQ=255) cTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACttt > 2:872301/1‑90 (MQ=255) gTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTgg > 2:1177331/1‑90 (MQ=255) aaaCAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAg < 1:1177331/90‑1 (MQ=255) aacaacGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGc < 1:1204756/90‑1 (MQ=255) cctgcTTTATCTCCCTGCGGAAGTGAAAATTCATCATAATGCCAGTGGTTCCTTTCTCGGGTTGGAGCCCCCCGAGCCGTTAGATGCCgg < 2:918030/89‑1 (MQ=255) ctgcGTTATCTGCCTGGGGAAGGGGAAATTCATAATAATGACAGTGTTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGc < 1:872301/90‑1 (MQ=255) ctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTGGATGCCGGc < 2:600347/90‑1 (MQ=255) ctgcctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGc > 1:117391/1‑90 (MQ=255) cctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTg > 1:909220/1‑90 (MQ=255) cctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTg > 2:162820/1‑90 (MQ=255) gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggcg > 2:423372/1‑90 (MQ=255) gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggcg > 1:546672/1‑90 (MQ=255) gtaaaaaTTCATCATAATGACAGTGTTTCTTTTCTTTGTTTGGACCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTg < 2:562572/87‑1 (MQ=255) | GCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTG > NZ_CP009273/1505343‑1505516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |