| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 115,628 | A→C | T137P (ACG→CCG) | ampD → | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 115,628 | 0 | A | C | 98.0% | 137.9 / NA | 51 | T137P (ACG→CCG) | ampD | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (27/23); minor base T (1/0); total (28/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCC > NZ_CP009273/115542‑115714 | gCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCcgc > 2:487515/1‑90 (MQ=255) tGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGc < 2:769602/90‑1 (MQ=255) aTTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCAc < 1:440404/90‑1 (MQ=255) ttttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACtg > 2:490926/1‑90 (MQ=255) cTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACtgattg < 1:511007/90‑1 (MQ=255) aTTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACt > 1:119047/1‑83 (MQ=255) aTTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACt < 2:119047/83‑1 (MQ=255) ttGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTgattgatt > 1:999385/1‑90 (MQ=255) ttGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTgattgatt < 1:1133991/90‑1 (MQ=255) ttGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTgattgatt < 1:709927/90‑1 (MQ=255) gggATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGc < 1:205709/90‑1 (MQ=255) tGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGATTGCTATcc > 2:873475/1‑90 (MQ=255) tgtaGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGatat < 2:876547/87‑1 (MQ=255) tGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGatat < 2:495261/90‑1 (MQ=255) tGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGatat < 1:979965/90‑1 (MQ=255) tGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACCGATTGATTTCTATCCGGatat > 1:287297/1‑90 (MQ=255) gAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGGTATc > 2:122739/1‑90 (MQ=255) cACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTaa > 2:549003/1‑90 (MQ=255) gATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGTTTGGTTGCTATCCGGATATCGCTAAAAAc > 1:598525/1‑90 (MQ=255) gATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAc < 2:1222510/90‑1 (MQ=255) gCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTTTCCGGGTATCGCTAAAAAAATGag > 2:998069/1‑89 (MQ=255) cTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCCCGGGCACTGATTGATTTCTATCCGGATATCGCTAAAAAAATGACg > 1:785767/1‑90 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 2:450450/90‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 1:1034530/90‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 1:1125907/90‑1 (MQ=255) gTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGATTGCTATCCGGGTATCGCTAAAAAAATGACggggcc > 2:147432/1‑87 (MQ=255) tataCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt < 1:761292/90‑1 (MQ=255) tataCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt < 2:822445/90‑1 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGCCTGATTGATTTCTATCCGGATATTGCTAAAAAAATGACGGGCCATTGTGat > 1:683426/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGGTTGCTATCCGGATATCGCTAAAAACATGACGGGCCCTtgtggt > 1:1021833/1‑88 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGat > 1:1067087/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGat > 2:503522/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGat > 2:312644/1‑90 (MQ=255) gcgcAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGGTTGGTATCCGGGTATCGCTAAAAACATGAAGGGGCATTTTGGTAtt > 2:622319/1‑90 (MQ=255) gcgcAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCCGATATCGCTAAAAAAATGACGGGCCCTTGTGATAtt > 2:124720/1‑90 (MQ=255) gcgcAGTATCAACAGCTTGCGGCGGTTCCCCGGGCACTGGTTTATTGCTATCCGGATATCGCTAAAAACATGACGGGGCATTTTGATAtt > 2:670963/1‑90 (MQ=255) gcAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTgc > 2:577262/1‑90 (MQ=255) gTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGatat < 2:305697/54‑1 (MQ=255) gTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGatat > 1:305697/1‑54 (MQ=255) tttGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGGTATCGCTAAAAACATGACGGGGCATTTTGATATTGCGCCGGATCGGaaa > 2:673346/2‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCCCTGATTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGGCCTTGTGATATTGCGCCGGGTCGGaaa > 1:698965/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATc < 2:909233/37‑1 (MQ=39) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATc > 1:909233/1‑37 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGGGATATTTCGCCGGATCGGaaa > 2:30035/1‑90 (MQ=255) cTTGCGGCGGTTCCCCGGGCACTGATTTATTTCTATCCGGATATCGCTAAAAAAATGACGGGCCATTGTGATATTTCGCCGGATCGGaaa > 1:697590/1‑90 (MQ=255) cTTGCGGCGGGTTCGCGGGGACTGGTTGATTTTTATCCGGATATCGCTAAAAAAATGAAGGGGCATTTTGATTTTTCGCCCGGTCGGaaa > 1:1067137/1‑90 (MQ=255) tGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAAc < 1:587632/90‑1 (MQ=255) cggcggTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCg < 1:621828/90‑1 (MQ=255) gcggTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGAt < 2:114607/90‑1 (MQ=255) gTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATccc < 1:607403/90‑1 (MQ=255) gTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATccc < 2:1067137/90‑1 (MQ=255) | GCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCC > NZ_CP009273/115542‑115714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |